1313488-63-9Relevant academic research and scientific papers
Fast copper-free click DNA ligation by the ring-strain promoted alkyne-azide cycloaddition reaction
Shelbourne, Montserrat,Chen, Xiong,Brown, Tom,El-Sagheer, Afaf H.
, p. 6257 - 6259 (2011)
Templated DNA strand ligation by the ring-strain promoted alkyne-azide [3+2] cycloaddition reaction is very fast; with dibenzocyclooctyne, the reaction is essentially complete in 1 min. It is inhibited by the presence of a single mismatched base pair sugg
OLIGONUCLEOTIDE LIGATION
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Paragraph 0128-0132, (2013/09/12)
A method for joining oligonucleotides. The method includes joining together one or more oligonucleotides by reacting an alkyne group lined to an oligonucleotide with an azide group linked to an oligonucleotide to form a triazole linkage. The alkyne group is a strained alkyne group. The method can include ligating together ends of one or more oligonucleotides or cross-linking strands of an oligonucleotide duplex. The methods described allow oligonucleotide strands to be ligated together without the need for a ligase enzyme.
