133-92-6Relevant articles and documents
The last step of kanamycin biosynthesis: Unique deamination reaction catalyzed by the α-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK
Sucipto, Hilda,Kudo, Fumitaka,Eguchi, Tadashi
supporting information; experimental part, p. 3428 - 3431 (2012/06/30)
Mystery solved: Using heterologous expression, the activities of two enzymes exclusively belonging to the kanamycin biosynthetic pathway have been identified in vitro. A distinctive reaction mechanism (see scheme) to produce kanamycin is proposed and the previously unknown catalytic deamination activity of KanJ dioxygenase is uncovered. Copyright