Analysis of KANAMYCIN SULFATE (cas 133-92-6) by liquid chromatography with pulsed electrochemical detection

Add time:09/29/2019    Source:infona.pl

The analysis of KANAMYCIN SULFATE (cas 133-92-6) by liquid chromatography using a column packed with poly(styrene-divinylbenzene) and pulsed electrochemical detection on a gold electrode is described. A two-step gradient was necessary to obtain a good separation together with a reasonable analysis time of maximum 45 min. The mobile phases consisted of an aqueous solution of 20 g/l or 60 g/l sodium sulfate, 1.3 g/l sodium octanesulfonate and 50 ml/l 0.2 M phosphate buffer pH 3.0. Sodium hydroxide was added post-column. The influence of the different chromatographic parameters on the separation was investigated. A number of commercial samples were analyzed using this method. Besides the previously reported impurities, such as kanamycins B and C, two other impurities were separated, one of which is called kanamycin D. In total, eight components were separated.

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