13322-96-8

Basic information

• Product Name: BETA-D-FRUCTOFURANOSYL-ALPHA-D-GALACTOPYRANOSIDE
• Synonyms: BETA-D-FRUCTOFURANOSYL-ALPHA-D-GALACTOPYRANOSIDE;SS-D-FRUCTOFURANOSYL-ALPHA-D-GALACTOPYRANOSIDE;b-D-Fructofuranosyl-a-D-galactopyranoside
• CAS NO: 13322-96-8
• Molecular Formula: C₁₂H₂₂O₁₁
• Molecular Weight: 342.29648
• Mol File: 13322-96-8.mol
• Article Data: 8

Chemical Properties

• Melting Point: 179 °C
• Boiling Point: 697.1±55.0 °C(Predicted)
• Appearance: /
• Density: 1.77±0.1 g/cm3(Predicted)
• PKA: 12.81±0.70(Predicted)
• CAS DataBase Reference: BETA-D-FRUCTOFURANOSYL-ALPHA-D-GALACTOPYRANOSIDE(CAS DataBase Reference)
• NIST Chemistry Reference: BETA-D-FRUCTOFURANOSYL-ALPHA-D-GALACTOPYRANOSIDE(13322-96-8)
• EPA Substance Registry System: BETA-D-FRUCTOFURANOSYL-ALPHA-D-GALACTOPYRANOSIDE(13322-96-8)

Safety Data

• Hazardous Substances Data: 13322-96-8(Hazardous Substances Data)

Upstream product

• 59-23-4 D-Galactose 
• 512-69-6 D-raffinose 
• 90699-76-6 1,3,4,6-tetra-O-pivaloyl-β-D-fructofuranosyl 4-O-benzoyl-2,3,6-tri-O-pivaloyl-α-D-galactopyranoside 
• 57-50-1 Sucrose 
• 10257-28-0 D-Galactose 
• 90653-38-6 C₄₈H₈₀O₂₀S 
• 69075-32-7 1,3,4,6-tetra-O-pivaloyl-β-D-fructofuranosyl 2,3,6-tri-O-pivaloyl-α-D-glucopyranoside 
• 60825-18-5 4,6-O-Isopropylidensucrose-hexaacetat 
• 52706-47-5 1',2,3,3',4',6'-hexa-O-acetyl sucrose 
• 862574-94-5 Acetic acid (2S,3S,4R,5R)-4-acetoxy-2,5-bis-acetoxymethyl-2-((2R,3R,4R,5R,6R)-3,4-diacetoxy-5-trifluoromethanesulfonyloxy-6-trifluoromethanesulfonyloxymethyl-tetrahydro-pyran-2-yloxy)-tetrahydro-furan-3-yl ester 
• 7296-64-2 β-D-galactopyranoside 
• 40039-10-9 1,3,4,6-tetra-O-acetyl-β-D-fructofuranosyl 2,3,4,6-tetra-O-acetyl-α-D-galactopyranoside 
• 470-23-5 D-fructose 

Downstream Products

• 7013-25-4 β-D-glucopyranosyl-(1→6)-arbutin 

Relevant articles and documents

Probing substrate promiscuity of amylosucrase from neisseria polysaccharea

The amylosucrase from Neisseria polysaccharea (NpAS) naturally catalyzes the synthesis of a variety of products from sucrose and shows signs of plasticity of its active site. To explore further this promiscuity, the tolerance of amylosucrase towards different donor and acceptor substrates was investigated. The selection of alternate donor substrates was first made on the basis of preliminary molecular modeling studies. From 11 potential donors harboring selective derivatizations that were experimentally evaluated, only p-nitrophenyl-α-D-glucopyranoside was used by the wild-type enzyme, and this underlines the high specificity of the -1 subsite of NpAS for glucosyl donor substrates. The acceptor substrate promiscuity was further explored by screening 20 hydroxylated molecules, including D- and L-monosaccharides as well as polyols. With the exception of one compound, all were successfully glucosylated, and this showcases the tremendous plasticity of the +1 subsite of NpAS, which is responsible for acceptor recognition. The products obtained from the transglucosylation reactions of three selected acceptors were characterized, and they revealed original structures and enzyme enantiopreference, which were more particularly analyzed by insilico docking analyses.

Method for synthesizing oligosaccharides and glycosylation

The invention relates to an enzymatic method for synthesizing oligosaccharides, whereby one saccharide group of a sucrose analogue each is transferred onto an acceptor molecule, for example for glycosylating a hydroxyl compound, a saccharide, peptide, or a drug. According to the inventive method, an enzymatic synthesis of β-D-fructofuranosyl-a-D-aldopyranoside is carried out in a first step, and in a second step one of the saccharide groups is enzymatically transferred onto the acceptor molecule.

Biocatalytic and chemical investigations in the synthesis of sucrose analogues

Herein, we report about the synthesis of sucrose analogues, obtained by two different approaches: a chemical and an enzymatic. The one step synthesis of the sucrose analogues with the exo-fructosyltransferase (EC 2.4.1.162) from Bacillus subtilis NCIMB 11871, which transfers the fructosyl residue of the substrate sucrose to the monosaccharide acceptors galactose, mannose, xylose and fucose, has been developed. Effects in the fructosylation by variation of the positions of the hydroxyl-groups in glycopyranoside acceptors have been studied in respect to their acceptor properties. In contrast, the chemical equivalent nonenzymatic organic synthesis of galacto-sucrose and manno-sucrose has been achieved including six synthetic steps.