1332729-29-9

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 10280-24-7 6-N-benzoyl-9-(2,3-O-ethoxymethylidene-β-D-ribofuranosyl)adenine 

Downstream Products

• 1332729-41-5 4-methoxy-1-oxido-2-pyridylmethyl [1-(6-N-benzoyladenin-9-yl)-2-O-benzoyloxymethoxymethyl-3-O-dimethoxytrityl-β-D-ribofuranos-5-O-yl]methylphosphonate 

Relevant academic research and scientific papers

Activation of human RNase L by 2′- and 5′-O-methylphosphonate- modified oligoadenylates

To determine the influence of internucleotide linkage and sugar ring conformation, and the role of 5′-terminal phosphate, on the activation of human RNase L, a series of 2′- and 5′-O-methylphosphonate-modified tetramers were synthesized from appropriate m

Synthesis of oligoribonucleotides with phosphonate-modified linkages

Solid phase synthesis of phosphonate-modified oligoribonucleotides using 2′-O-benzoyloxymethoxymethyl protected monomers is presented in both 3′→5′ and 5′→3′ directions. Hybridisation properties and enzymatic stability of oligoribonucleotides modified by regioisomeric 3′- and 5′-phosphonate linkages are evaluated. The introduction of the 5′-phosphonate units resulted in moderate destabilisation of the RNA/RNA duplexes (ΔTm -1.8 °C/mod.), whereas the introduction of the 3′-phosphonate units resulted in considerable destabilisation of the duplexes (ΔTm -5.7 °C/mod.). Molecular dynamics simulations have been used to explain this behaviour. Both types of phosphonate linkages exhibited remarkable resistance in the presence of ribonuclease A, phosphodiesterase I and phosphodiesterase II.