1335135-87-9Relevant academic research and scientific papers
Conventional and microwave-assisted SPPS approach: A comparative synthesis of PTHrP(1-34)NH2
Rizzolo, Fabio,Testa, Chiara,Lambardi, Duccio,Chorev, Michael,Chelli, Mario,Rovero, Paolo,Papini, Anna Maria
experimental part, p. 708 - 714 (2012/05/20)
Attracted by the possibility to optimize time and yield of the synthesis of difficult peptide sequences by MW irradiation, we compared Fmoc/tBu MW-assisted SPPS of 1-34 N-terminal fragment of parathyroid hormone-related peptide (PTHrP) with its conventional SPPS carried out at RT. MWs were applied in both coupling and deprotection steps of SPPS protocol. During the stepwise elongation of the resin-bound peptide, monitoring was conducted by performing MW-assisted mini-cleavages and analyzing them by UPLC-ESI-MS. Identification of some deletion sequences was helpful to recognize critical couplings and as such helped to guide the introduction of MW irradiations to these stages.
