1350820-80-2Relevant academic research and scientific papers
Design, synthesis, and incorporation of fluorous 5-methylcytosines into oligonucleotides
Song, Zhiquan,Zhang, Qisheng
, p. 10263 - 10268 (2012/02/13)
A palladium-catalyzed Negishi coupling reaction has been developed to synthesize fluorous 5-methyl-cytosines. These fluorous nucleosides are incorporated into the oligonucleotides that correspond to part of the promoter region of Oct4, a master gene that undergoes dynamic DNA demethylation during cellular reprogramming. The separation of the fluorous oligonucleotides from its nonfluorous analogues has been achieved through solid-phase extraction over fluorous silica, suggesting its potential use in probing DNA demethylation (Figure presented).
