1350928-29-8Relevant articles and documents
The development of a fluorescence turn-on sensor for cysteine, glutathione and other biothiols. A kinetic study
García-Beltrán, Olimpo,Mena, Natalia,Pérez, Edwin G.,Cassels, Bruce K.,Nu?ez, Marco T.,Werlinger, Francisca,Zavala, Daniel,Aliaga, Margarita E.,Pavez, Paulina
, p. 6606 - 6609 (2012/01/06)
Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols toward both probes (ChC1 and ChC2) follows the order Cys > GSH > Hcy > Cys-Gly, ChC1 being less reactive than ChC2. Possible interference with other amino acids was assessed. ChC1 and ChC2 display a highly selective fluorescence enhancement with thiols, allowing these probes to be used for fluorimetric thiol determination in SH-SY5Y cells.