1353015-41-4Relevant academic research and scientific papers
New rhodamine nitroxide based fluorescent probes for intracellular hydroxyl radical identification in living cells
Yapici, Nazmiye B.,Jockusch, Steffen,Moscatelli, Alberto,Mandalapu, Srinivas Rao,Itagaki, Yasuhiro,Bates, Dallas K.,Wiseman, Sherri,Gibson, K. Michael,Turro, Nicholas J.,Bi, Lanrong
, p. 50 - 53 (2012)
The synthesis, characteristics, and biological applications of a series of new rhodamine nitroxide fluorescent probes that enable imaging of hydroxyl radicals (?OH) in living cells are described. These probes are highly selective for ?OH in aqueous solution, avoiding interference from other reactive oxygen species (ROS), and they facilitate ?OH imaging in biologically active samples. The robust nature of these probes (high specificity and selectivity, and facile synthesis) offer distinct advantages over previous methods for ?OH detection.
Determination of intracellular pH using sensitive, clickable fluorescent probes
Yapici, Nazmiye B.,Mandalapu, Srinivas Rao,Chew, Teng-Leong,Khuon, Satya,Bi, Lanrong
, p. 2440 - 2443 (2012/05/19)
We synthesized and evaluated a series of acidic fluorescent pH probes exhibiting robust pH dependence, high sensitivity and photostability, and excellent cell membrane permeability. Titration analyses indicated that probe 3 could increase its fluorescence intensity 800-fold between pH 8.0 and 4.1. Additionally, its pKa value is optimal for intracellular probing of acidic organelles. Fluorescent imaging of HepG2 and Hela cells further revealed that probe 3 demonstrates outstanding capacity for monitoring of intracellular [H+] levels. The easily accessible terminal alkyne/azido function groups of these probes offer the possibility of rapidly constructing sensor molecule libraries using 'click' chemistry.
