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Z-GLY-GLY-ONP is a chemical compound consisting of two glycine molecules linked by a peptide bond with a cis configuration, indicated by the Z prefix. Z-GLY-GLY-ONP features an O-nitrophenyl (ONP) group attached at the N-terminus, which enables spectrophotometric detection due to its light-absorbing properties. This characteristic makes Z-GLY-GLY-ONP a valuable tool in biochemistry and enzymology for the study of enzymatic activity, specificity, and inhibition.

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  • 13574-81-7 Structure
  • Basic information

    1. Product Name: Z-GLY-GLY-ONP
    2. Synonyms: N-CBZ-GLYCYLGLYCINE P-NITROPHENYL ESTER;N-CBZ-GLY-GLY P-NITROPHENYL ESTER;N-CARBOBENZOXY-GLYCYLGLYCINE-P-NITROPHENYL ESTER;Z-GLY-GLY-ONP;Z-GLYCYL-GLYCINE 4-NITROPHENYL ESTER;Z-GLYCYL-GLYCINE P-NITROPHENYL ESTER;N-CBZ-Glycylglycine p-nitrophenyl ester N-Carbobenzyloxy-glycylglycine p-nitrophenyl ester;z-gly-gly p-nitrophenyl ester
    3. CAS NO:13574-81-7
    4. Molecular Formula: C18H17N3O7
    5. Molecular Weight: 387.34
    6. EINECS: N/A
    7. Product Categories: Dipeptides;Dipeptides and Tripeptides;Peptides
    8. Mol File: 13574-81-7.mol
  • Chemical Properties

    1. Melting Point: 161-163 °C
    2. Boiling Point: 676.4 ºCat 760 mmHg
    3. Flash Point: 362.9 ºC
    4. Appearance: /
    5. Density: 1.364 g/cm3
    6. Vapor Pressure: 3.75E-18mmHg at 25°C
    7. Refractive Index: 1.592
    8. Storage Temp.: -15°C
    9. Solubility: N/A
    10. PKA: 10.98±0.46(Predicted)
    11. CAS DataBase Reference: Z-GLY-GLY-ONP(CAS DataBase Reference)
    12. NIST Chemistry Reference: Z-GLY-GLY-ONP(13574-81-7)
    13. EPA Substance Registry System: Z-GLY-GLY-ONP(13574-81-7)
  • Safety Data

    1. Hazard Codes: N
    2. Statements: 50/53
    3. Safety Statements: 60-61
    4. RIDADR: UN 3077 9/PG 3
    5. WGK Germany:
    6. RTECS:
    7. HazardClass: N/A
    8. PackingGroup: N/A
    9. Hazardous Substances Data: 13574-81-7(Hazardous Substances Data)

13574-81-7 Usage

Uses

Used in Biochemistry and Enzymology:
Z-GLY-GLY-ONP is used as a chromogenic substrate for various peptidases and proteases. The presence of the ONP group allows for the quantitative measurement of enzymatic reactions through spectrophotometry, providing insights into enzyme activity, specificity, and the potential for inhibitor development.
Used in Pharmaceutical Research:
In the pharmaceutical industry, Z-GLY-GLY-ONP can be utilized for the development of enzyme inhibitors and the study of enzyme mechanisms. Its ability to be easily detected and quantified makes it a useful probe for high-throughput screening of potential enzyme inhibitors and for understanding the kinetics and dynamics of enzymatic reactions.
Used in Diagnostic Applications:
Z-GLY-GLY-ONP can be employed in diagnostic assays to detect and measure the activity of specific peptidases and proteases, which may be relevant to certain diseases or conditions. Z-GLY-GLY-ONP's spectrophotometric properties facilitate the development of sensitive and specific diagnostic tests.
Used in Educational Settings:
In academic and educational environments, Z-GLY-GLY-ONP serves as a practical tool for teaching enzyme kinetics, mechanisms, and the use of spectrophotometry in biochemical analysis. Its straightforward structure and detectable properties make it an ideal substrate for hands-on laboratory exercises and demonstrations.

Check Digit Verification of cas no

The CAS Registry Mumber 13574-81-7 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,3,5,7 and 4 respectively; the second part has 2 digits, 8 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 13574-81:
(7*1)+(6*3)+(5*5)+(4*7)+(3*4)+(2*8)+(1*1)=107
107 % 10 = 7
So 13574-81-7 is a valid CAS Registry Number.
InChI:InChI=1/C18H17N3O7/c22-16(10-20-18(24)27-12-13-4-2-1-3-5-13)19-11-17(23)28-15-8-6-14(7-9-15)21(25)26/h1-9H,10-12H2,(H,19,22)(H,20,24)

13574-81-7SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 17, 2017

Revision Date: Aug 17, 2017

1.Identification

1.1 GHS Product identifier

Product name (4-nitrophenyl) 2-[[2-(phenylmethoxycarbonylamino)acetyl]amino]acetate

1.2 Other means of identification

Product number -
Other names Z-Gly-Gly-OnPh

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:13574-81-7 SDS

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