136044-34-3

Basic information

• Product Name: N⁶-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)adenosine 3'-O-(2-cyanoethyl N,N-diethylphosphoramidite)
• Synonyms: N⁶-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)adenosine 3'-O-(2-cyanoethyl N,N-diethylphosphoramidite)
• CAS NO: 136044-34-3
• Molecular Weight: 960.155
• Mol File: 136044-34-3.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: N⁶-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)adenosine 3'-O-(2-cyanoethyl N,N-diethylphosphoramidite)(CAS DataBase Reference)
• NIST Chemistry Reference: N⁶-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)adenosine 3'-O-(2-cyanoethyl N,N-diethylphosphoramidite)(136044-34-3)
• EPA Substance Registry System: N⁶-benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)adenosine 3'-O-(2-cyanoethyl N,N-diethylphosphoramidite)(136044-34-3)

Safety Data

• Hazardous Substances Data: 136044-34-3(Hazardous Substances Data)

Upstream product

• 110914-51-7 chloro-β-cyanoethyl-N,N-diisopropylamino-phosphoramidite 
• 129681-71-6 5'-O-dimethoxytrityl-2'-O-tert-butyldimethylsilyl-N₆-benzoyl adenosine 

Relevant articles and documents

New Nucleoside Phosphoramidites and Coupling Protocols for Solid-Phase RNA Synthesis

The 5'-O-(4,4'-dimethoxytrityl)-2'-O-(trialkylsilyl)ribonucleoside 3'-O-(2-cyanoethyl N,N-diethylphosphoramidites) 3, 5, 7, and 9, modified monomers for RNA synthesis, were prepared from 2-cyanoethyl N,N-diethylchlorophosphoramidite (1).In conjunction with newly developed coupling protocols for automated solid-phase synthesis, they afforded synthetic oligoribonucleotides up to 74 base units in length.The performance of the new compounds was compared to the analogous 5'-O-(4,4'-dimethoxytrityl)-2'-O-(trialkylsilyl)ribonucleoside 3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) 4, 6, 8, and 10.Complete removal of benzoyl groups from N2-benzoylguanosine, which was incorporated into some of the synthetic oligoribonucleotides, was demonstrated.Purification procedures by reverse phase HPLC and PAGE methods are also presented.