136765-45-2Relevant articles and documents
Preparation method of stable isotope labeled nitrazepam internal standard reagent
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, (2021/10/16)
The invention relates to a preparation method of a stable isotope labeled nitrazepam internal standard reagent for food safety detection, and belongs to the field of research and development of standard substances for food safety detection. The stable isotope labeled nitrazepam is obtained from stable isotope labeled phenylboronic acid as a raw material through catalytic addition, acylation, cyclization and final separation and purification. The process has the advantages that the raw material required for synthesis is simple and easy to obtain, the operation is simple, the reaction conditions are mild, the final product is high in chemical purity, isotope abundance is not diluted, toxic and carcinogenic chemical reagents are not used, and the risk of personnel health and environmental pollution is effectively reduced. The chemical purity of the stable isotope labeled nitrazepam prepared by the preparation method can reach 98% or above, the isotope abundance can reach 98% or above, and the technical requirements of a stable isotope labeled internal standard reagent required by a liquid chromatography-tandem mass spectrometry method for food safety detection can be met.