1374570-29-2Relevant academic research and scientific papers
A semisynthetic fluorescent sensor protein for glutamate
Brun, Matthias A.,Tan, Kui-Thong,Griss, Rudolf,Kielkowska, Anna,Reymond, Luc,Johnsson, Kai
, p. 7676 - 7678 (2012/07/02)
We report the semisynthesis of a fluorescent glutamate sensor protein on cell surfaces. Sensor excitation at 547 nm yields a glutamate-dependent emission spectrum between 550 and 700 nm that can be exploited for ratiometric sensing. On cells, the sensor displays a ratiometric change of 1.56. The high sensitivity toward glutamate concentration changes of the sensor and its exclusive extracellular localization make it an attractive tool for glutamate sensing in neurobiology.
