1381777-82-7

Basic information

• Product Name: p-(α-azidoacetamido)phenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside
• Synonyms: p-(α-azidoacetamido)phenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside
• CAS NO: 1381777-82-7
• Molecular Weight: 522.469
• Mol File: 1381777-82-7.mol
• Article Data: 4

Chemical Properties

• CAS DataBase Reference: p-(α-azidoacetamido)phenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside(CAS DataBase Reference)
• NIST Chemistry Reference: p-(α-azidoacetamido)phenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside(1381777-82-7)
• EPA Substance Registry System: p-(α-azidoacetamido)phenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside(1381777-82-7)

Safety Data

• Hazardous Substances Data: 1381777-82-7(Hazardous Substances Data)

Upstream product

• 3150-24-1 4-nitrophenyl-β-D-galactopyranoside 
• 2872-66-4 4-nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside 
• 42011-36-9 (2R,3S,4S,5R,6S)-2-(acetoxymethyl)-6-(4-aminophenoxy)tetrahydro-2H-pyran-3,4,5-triyl triacetate 
• 4163-60-4 β-D-galactose peracetate 
• 100-02-7 4-nitro-phenol 

Downstream Products

• 1381777-84-9 4-[(1,2,3-triazol-4-hydroxymethyl-1-yl)-acetamido]phenyl β-D-galactopyranoside 
• 1381777-83-8 4-[(1,2,3-triazol-4-acetoxymethyl-1-yl)-acetamido]phenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside 
• 1448147-94-1 3-{1-[N-{4-(2,3,4,6-tetra-O-acetyl-β-D-galactopyranosyloxy)phenyl}carbamoylmethyl]-1,2,3-triazol-4-yl}propionoic acid succinimidyl ester 

Relevant articles and documents

Development and optimization of a competitive binding assay for the galactophilic low affinity lectin LecA from: Pseudomonas aeruginosa

Infections with the Gram-negative bacterium Pseudomonas aeruginosa result in a high mortality among immunocompromised patients and those with cystic fibrosis. The pathogen can switch from planktonic life to biofilms, and thereby shields itself against antibiotic treatment and host immune defense to establish chronic infections. The bacterial protein LecA, a C-type lectin, is a virulence factor and an integral component for biofilm formation. Inhibition of LecA with its carbohydrate ligands results in reduced biofilm mass, a potential Achilles heel for treatment. Here, we report the development and optimization of a fluorescence polarization-based competitive binding assay with LecA for application in screening of potential inhibitors. As a consequence of the low affinity of d-galactose for LecA, the fluorescent ligand was optimized to reduce protein consumption in the assay. The assay was validated using a set of known inhibitors of LecA and IC50 values in good agreement with the known Kd values were obtained. Finally, we employed the optimized assay to screen sets of synthetic thio-galactosides and natural blood group antigens and report their structure-activity relationship. In addition, we evaluated a multivalent fluorescent assay probe for LecA and report its applicability in an inhibition assay.

Rational design and synthesis of optimized glycoclusters for multivalent lectin-carbohydrate interactions: Influence of the linker arm

The design of multivalent glycoclusters requires the conjugation of biologically relevant carbohydrate epitopes functionalized with linker arms to multivalent core scaffolds. The multigram-scale syntheses of three structurally modified triethyleneglycol analogues that incorporate amide moiety(ies) and/or a phenyl ring offer convenient access to a series of carbohydrate probes with different water solubilities and rigidities. Evaluation of flexibility and determination of preferred conformations were performed by conformational analysis. Conjugation of the azido-functionalized carbohydrates with tetra-propargylated core scaffolds afforded a library of 18 tetravalent glycoclusters, in high yields, by CuI-catalyzed azide-alkyne cycloaddition (CuAAC). The compounds were evaluated for their ability to bind to PA-IL (the LecA lectin from the opportunistic pathogen Pseudomonas aeruginosa). Biochemical evaluation through inhibition of hemagglutination assays (HIA), enzyme-linked lectin assays (ELLA), surface plasmon resonance (SPR), and isothermal titration microcalorimetry (ITC) revealed improved and unprecedented affinities for one of the monovalent probes (Kd=5.8 μM) and also for a number of the tetravalent compounds that provide several new nanomolar ligands for this tetrameric lectin. Copyright