1399358-40-7Relevant academic research and scientific papers
Introduction of a non-natural amino acid into a nonribosomal peptide antibiotic by modification of adenylation domain specificity
Thirlway, Jenny,Lewis, Richard,Nunns, Laura,Al Nakeeb, Majid,Styles, Matthew,Struck, Anna-Winona,Smith, Colin P.,Micklefield, Jason
experimental part, p. 7181 - 7184 (2012/08/28)
Calcium-dependent antibiotics (CDA) are cyclic lipopeptides assembled by nonribosomal peptide synthetase (NRPS) enzymes. Active site modification of the 3-methyl glutamate activating adenylation (A) domain of the CDA NRPS enables the incorporation of synthetic 3-methyl glutamine into CDA. This provides the first example of how A-domains can be engineered to introduce synthetic non-natural amino acids into nonribosomal peptides. Copyright
