1402544-65-3

Upstream product

• 622-79-7 benzyl azide 
• 1402544-60-8 6-{4-[1-benzyl-1H-(1,2,3)-triazole-4-yl]butyl}-3-(2-deoxy-β-D-erythropentofuranosyl)pyrrolo[2,3-d]pyrimidin-2(3H)one 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 1033295-11-2 3-(2-deoxy-β-D-erythro-pentofuranosyl)-3,7-dihydro-6-hexynyl-2H-pyrrolo[2,3-d]pyrimidin-2-one 

Downstream Products

• 1402544-66-4 6-{4-[1-benzyl-1H-(1,2,3)-triazole-4-yl]butyl}-3-(2-deoxy-5-O-(4,4'-dimethoxytriphenylmethyl)-β-D-erythropentofuranosyl)pyrrolo[2,3-d]pyrimidin-2(3H)one 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite 

Relevant academic research and scientific papers

A nucleobase-discriminating pyrrolo-dC click adduct designed for DNA fluorescence mismatch sensing

New pyrrolo-dC click adducts (4 and 5) tethered with a 1,2,3-triazole skeleton were synthesized and oligonucleotides were prepared. The triazole system was either directly linked to the pyrrolo moiety (5) or connected via an n-butyl linker (4). The quantum yield of nucleoside 5 (φ=0.32), which is 10 times higher than those of 8-methylpyrrolo-dC (1 b, φ=0.026) or the long linker derivative 4 (φ=0.03), is maintained in oligonucleotides. Compound 5 was used as a nucleobase-discriminating fluorescence sensor in duplex DNA. Excellent mismatch discrimination was observed when 5 was positioned opposite the four canonical nucleosides. Compound 5 has the potential to be used for SNP detection in long DNA targets when conventional techniques such as high resolution melt analysis fail. Nucleobase analogues: Compound 1 (see scheme) was designed to act as a nucleobase-discriminating fluorescence sensor for duplex DNA. Excellent mismatch discrimination was observed when nucleoside 1 was positioned opposite the four canonical nucleosides within the DNA duplex. Copyright