1402832-60-3Relevant academic research and scientific papers
Synthesis of novel tetrazole C5-linked C0- and C 2-ribonucleoside phosphoramidites using MePOM and POM groups for probing RNA catalysis
Harusawa, Shinya,Yoneyama, Hiroki,Fujisue, Daiki,Nishiura, Masayoshi,Fujitake, Mihoyo,Usami, Yoshihide,Zhao, Zheng-Yun,Mcphee, Scott A.,Wilson, Timothy J.,Lilley, David M. J.
, p. 5891 - 5894,4 (2012)
Novel C5-linked C0- and C2-tetrazole ribonucleoside phosphoramidites were designed and synthesized via tetrazole C-nucleosides. Pivaloyloxymethyl (POM) and methyl-substituted POM (MePOM) groups were introduced as N-protecting groups in the tetrazole ring that can be readily removed under mild basic conditions. The phosphoramidites were successfully incorporated into the VS ribozyme substrate and hence providing a chemogenetic approach to determine which nucleobases of ribozymes function as the acid or base, in the studies of ribozyme general acid and base catalysis.
