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Usage

Uses

Used in Research Applications:
Thymidine 5'-monophosphate is utilized as a research tool for studying DNA synthesis, repair mechanisms, and cell replication processes. It aids in understanding the fundamental biological processes that underpin genetic information transfer and integrity.
Used in Medical Applications:
In the medical field, Thymidine 5'-monophosphate is used as a precursor for the synthesis of nucleic acid-based drugs, which have potential applications in treating various diseases, including genetic disorders and certain types of cancer.
Used in Pharmaceutical Development:
As a key component in the development of nucleic acid-based therapeutics, Thymidine 5'-monophosphate contributes to the creation of drugs that can target specific genetic sequences, offering a new avenue for personalized medicine and targeted therapies.
Used in Cell Culture Media:
In cell culture applications, Thymidine 5'-monophosphate is incorporated into media formulations to support the growth and replication of cells in vitro. This is particularly important for research and commercial production of biological products, such as vaccines and therapeutic proteins.
Used in Diagnostic Applications:
Thymidine 5'-monophosphate can also be used in diagnostic assays to detect and measure DNA synthesis and repair activities, providing insights into cellular health and the effectiveness of potential therapeutic interventions.

Upstream product

• 83918-61-0 thymidine 5'-p-nitrophenylphosphate 
• 87367-14-4 thymidilyl(3'-5')thymidine 

Relevant academic research and scientific papers

Synthesis and stability of a 2′-O-[N-(aminoethyl)carbamoyl] methyladenosine-containing dinucleotide

Working towards the synthesis of 2′-O-[N-(aminoethyl)carbamoyl] methyl-modified di- and oligonucleotides, we have synthesised a protected 2′-O-[N-(aminoethyl)carbamoyl]methyl-modified adenosine where the modification is introduced in a convenient one-pot three-step procedure. The corresponding H-phosphonate building block was also synthesised, and from this intermediate, a 2′-O-[N-(aminoethyl)carbamoyl]methyl-containing dinucleotide could be made. We also performed studies on the chemical and enzymatic stability of this dinucleotide. The dinucleotide was subjected to different ammonolysis and other basic conditions, and HPLC analysis showed that the modification was intact to most conditions, but that there was some minor hydrolysis when NH3 (concd. aq.) was used at 55 °C. Under several other sets of conditions, including saturated NH3 in methanol, and ethylenediamine, the amide remained intact. Treatment of the dinucleotide with Phosphodiesterase I from Crotalus adamanteus venom and Phosphodiesterase II from bovine spleen showed that the N-(aminoethyl)carbamoylmethyl moiety gives the phosphodiester linkage substantial protection against enzymatic degradation; the phosphodiester was not degraded by PDE II at all after seven days. A 2′-O-[N-(aminoethyl)carbamoyl]methyl-modified adenosine and a corresponding dinucleotide were synthesised. Hydrolysis (1-2 %) was observed in concentrated aqueous NH3 at 55°C, but under several other sets of reaction conditions, the amide remained intact. The N-(aminoethyl) carbamoylmethyl moiety gave substantial protection against enzymatic degradation by nucleases from snake venom and bovine spleen. Copyright

Kinetic and Theoretical Studies on the Mechanism of Alkaline Hydrolysis of DNA

The reaction mechanism of alkaline hydrolysis of DNA has been investigated by kinetic analysis and density-functional-theory calculation. The rates of hydrolysis of thymidine 3′-monophosphate esters (including thymidylyl(3′-5′)thymidine (Tp-OT)) monotonically decrease as the leaving groups get poorer. According to the theoretical calculation in which the solvent effects are incorporated, no intermediate is formed in the course of the reaction. In the alkaline hydrolysis of the activated Tp-OT analogues having good leaving groups, the 3′,5′-cyclic monophosphate of thymidine is concurrently formed through the intramolecular attack by the 5′-alkoxide ion. In the hydrolysis of the native dinucleotide, however, this side reaction does not occur, since the transition state leading to the departure of its poor leaving group cannot be formed due to conformational restraint. These arguments are supported by the theoretical analysis on the hydrolysis of both dimethyl phosphate and its O(bridging)→S substituted analogue.

A New Approach for Chemical Phosphorylation of Oligonucleotides at the 5'-Terminus

A new efficient method for chemical 5'-phosphorylation of synthetic oligonucleotides is described.Accordingly, 2-cyanoethyl 3-(4,4'-dimethoxytrityloxy)-2,2-di(ethoxycarbonyl)propyl-1 N,N-diisopropyl phosphoramidite (1) was introduced as the 5'-terminal bu

EFFECT OF NUCLEOPHILIC CATALYST ON THE DEGRADATIVE OLIGOMERIZATION OF THYMIDINE 5'-p-NITROPHENYLPHOSPHATE

Degradative oligomerization of thymidine 5'-p-nitrophenylphosphate(PNP-pT) were studied in the presence of imidazole, its derivatives, and metal ions.PNP-pT was degraded to give several kinds of thymidylic acid oligomers such as pTpT, TppT, cyclic-pTpT.The linearities of the structures of the obtained oligomers depended on the linearities of the structures of imidazole derivatives.Metal ions favoured formation of the cyclic dimer.