14057-65-9

Basic information

• Product Name: THYMIDINE 5'-MONOPHOSPHATE
• Synonyms: T-5'-P;THYMIDYLIC ACID;THYMIDYLIC ACID [5'];THYMIDINE 5'-MONOPHOSPHATE;THYMIDINE-5'-MONOPHOSPHORIC ACID;DTMP;ANTI-EMP1 (N-TERM) antibody produced in rabbit;B4B
• CAS NO: 14057-65-9
• Molecular Formula: C₁₀H₁₃N₂O₈P
• Molecular Weight: 322.21
• Mol File: 14057-65-9.mol
• Article Data: 4

Chemical Properties

• Appearance: /
• Storage Temp.: −20°C
• CAS DataBase Reference: THYMIDINE 5'-MONOPHOSPHATE(CAS DataBase Reference)
• NIST Chemistry Reference: THYMIDINE 5'-MONOPHOSPHATE(14057-65-9)
• EPA Substance Registry System: THYMIDINE 5'-MONOPHOSPHATE(14057-65-9)

Safety Data

• WGK Germany: 3
• RTECS: XP2090000
• Hazardous Substances Data: 14057-65-9(Hazardous Substances Data)

Usage

General Description

Thymidine 5'-monophosphate is a nucleotide, which is a building block of DNA. It is composed of a thymine base, a ribose sugar, and a phosphate group. This chemical is essential for DNA synthesis and repair, as it provides the necessary components for the construction of the DNA molecule. Thymidine 5'-monophosphate plays a crucial role in cell replication and growth, and is also involved in the regulation of gene expression. It is used in research and medical applications as a precursor for the synthesis of nucleic acid-based drugs and as a component in cell culture media.

Upstream product

• 87367-14-4 thymidilyl(3'-5')thymidine 
• 83918-61-0 thymidine 5'-p-nitrophenylphosphate 

Relevant articles and documents

Synthesis and stability of a 2′-O-[N-(aminoethyl)carbamoyl] methyladenosine-containing dinucleotide

Working towards the synthesis of 2′-O-[N-(aminoethyl)carbamoyl] methyl-modified di- and oligonucleotides, we have synthesised a protected 2′-O-[N-(aminoethyl)carbamoyl]methyl-modified adenosine where the modification is introduced in a convenient one-pot three-step procedure. The corresponding H-phosphonate building block was also synthesised, and from this intermediate, a 2′-O-[N-(aminoethyl)carbamoyl]methyl-containing dinucleotide could be made. We also performed studies on the chemical and enzymatic stability of this dinucleotide. The dinucleotide was subjected to different ammonolysis and other basic conditions, and HPLC analysis showed that the modification was intact to most conditions, but that there was some minor hydrolysis when NH3 (concd. aq.) was used at 55 °C. Under several other sets of conditions, including saturated NH3 in methanol, and ethylenediamine, the amide remained intact. Treatment of the dinucleotide with Phosphodiesterase I from Crotalus adamanteus venom and Phosphodiesterase II from bovine spleen showed that the N-(aminoethyl)carbamoylmethyl moiety gives the phosphodiester linkage substantial protection against enzymatic degradation; the phosphodiester was not degraded by PDE II at all after seven days. A 2′-O-[N-(aminoethyl)carbamoyl]methyl-modified adenosine and a corresponding dinucleotide were synthesised. Hydrolysis (1-2 %) was observed in concentrated aqueous NH3 at 55°C, but under several other sets of reaction conditions, the amide remained intact. The N-(aminoethyl) carbamoylmethyl moiety gave substantial protection against enzymatic degradation by nucleases from snake venom and bovine spleen. Copyright

A New Approach for Chemical Phosphorylation of Oligonucleotides at the 5'-Terminus

A new efficient method for chemical 5'-phosphorylation of synthetic oligonucleotides is described.Accordingly, 2-cyanoethyl 3-(4,4'-dimethoxytrityloxy)-2,2-di(ethoxycarbonyl)propyl-1 N,N-diisopropyl phosphoramidite (1) was introduced as the 5'-terminal bu