140659-99-0Relevant articles and documents
Overproduction of CMP-sialic acid synthetase for organic synthesis
Liu, Jennifer Lin-Chun,Shen, Gwo-Jenn,Ichikawa, Yoshitaka,Rutan, James F.,Zapata, Gerardo,Vann, Willie F.,Wong, Chi-Huey
, p. 3901 - 3910 (2007/10/02)
The gene coding for Escherichia coli CMP-sialic acid synthetase (E.G. 2.7.7.43) was cloned and overexpressed in E. coli through a primer-directed polymerase chain reaction. Two plasmids were constructed to produce the native enzyme and a modified enzyme f