1412458-66-2Relevant academic research and scientific papers
Synthesis and photoactivity of a Pt(II) complex based on an o-nitrobenzyl-derived ligand
Liu, Di,Ma, Jialiu,Zhou, Wen,He, Weijiang,Guo, Zijian
, p. 198 - 203 (2012)
Development of photoactivatable platinum complexes was an attractive approach to improve the anticancer behavior of Pt-based agents. In this work, a new photolabile Pt(II) complex, NMPt, was prepared using a photosensitive compound, 2-hydroxy-2-(2-nitrophenyl)acetic acid (HNPAC). X-ray crystallographic data disclosed that Pt(II) center of NMPt adopts a square-planar geometry similar to that of nedaplatin. In NMPt molecule, the Pt(II) center is coordinated by two oxygen atoms of the hydroxyl and carboxyl groups in HNPAC ligand and two ammine ligands. The quick conversion of NMPt (100 μM) in PBS buffer upon irradiation at 365 nm was observed, displaying a distinct absorption bathochromic shift from 265 to 300 nm. The 1H NMR determination also confirmed the UV irradiation-induced conversion of NMPt complex. The CD spectroscopic determination revealed that NMPt is able to distort the conformation of CT-DNA only upon UV-irradiation. In addition, agarose gel analysis of plasmid pBR 322 DNA demonstrated that NMPt induces plasmid DNA nicking upon UV-irradiation with a distinct dose- and time-dependence. Preliminary results showed that the cytotoxicity of NMPt at lower concentration (5-20 μM) against human breast adenocarcinoma (MCF-7) cells is comparable to that of nedaplatin upon UV irradiation, while its cytotoxicity is very low without UV irradiation. Therefore, UV irradiation-induced DNA distortion and damage might be correlated to the photo-induced cytotoxicity enhancement for NMPt. This study provides an interesting approach for the exploration of photoactivatable Pt(II) complex as anticancer agent, although the photo-induced anticancer activity of NMPt remains to be improved.
