1426561-75-2Relevant academic research and scientific papers
Photoclickable dendritic molecular glue: Noncovalent-to-covalent photochemical transformation of protein hybrids
Uchida, Noriyuki,Okuro, Kou,Niitani, Yamato,Ling, Xiao,Ariga, Takayuki,Tomishige, Michio,Aida, Takuzo
supporting information, p. 4684 - 4687 (2013/05/09)
A water-soluble dendron with a fluorescein isothiocyanate (FITC) fluorescent label and bearing nine pendant guanidinium ion (Gu +)/benzophenone (BP) pairs at its periphery (GlueBP-FITC) serves as a photoclickable molecular glue . By multivalent salt-bridge formation between Gu+ ions and oxyanions, Glue BP-FITC temporarily adheres to a kinesin/microtubule hybrid. Upon subsequent exposure to UV light, this noncovalent binding is made permanent via a cross-linking reaction mediated by carbon radicals derived from the photoexcited BP units. This temporal-to-permanent transformation by light occurs quickly and efficiently in this preorganized state, allowing the movements of microtubules on a kinesin-coated glass plate to be photochemically controlled. A fundamental difference between such temporal and permanent bindings was visualized by the use of optical tweezers .
