1443369-41-2Relevant academic research and scientific papers
Selective detection of 5-formyl-2′-deoxycytidine in DNA using a fluorogenic hydroxylamine reagent
Guo, Pu,Yan, Shengyong,Hu, Jianlin,Xing, Xiwen,Wang, Changcheng,Xu, Xiaowei,Qiu, Xiaoyu,Ma, Wen,Lu, Chunjiang,Weng, Xiaocheng,Zhou, Xiang
, p. 3266 - 3269 (2013)
Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.
