1448147-76-9Relevant academic research and scientific papers
Synthesis of multivalent carbohydrate-centered glycoclusters as nanomolar ligands of the bacterial lectin LecA from pseudomonas aeruginosa
Gening, Marina L.,Titov, Denis V.,Cecioni, Samy,Audfray, Aymeric,Gerbst, Alexey G.,Tsvetkov, Yury E.,Krylov, Vadim B.,Imberty, Anne,Nifantiev, Nikolay E.,Vidal, Sebastien
supporting information, p. 9272 - 9285 (2013/07/26)
A family of fifteen glycoclusters based on a cyclic oligo-(1→6)- β-D-glucosamine core has been designed as potential inhibitors of the bacterial lectin LecA with various valencies (from 2 to 4) and linkers. Evaluation of their binding properties towards LecA has been performed by a combination of hemagglutination inhibition assays (HIA), enzyme-linked lectin assays (ELLA), and isothermal titration microcalorimetry (ITC). Divalent ligands displayed dissociation constants in the sub-micromolar range and tetravalent ligands displayed low nanomolar affinities for this lectin. The influence of the linker could also be demonstrated; aromatic moieties are the best scaffolds for binding to the lectin. Copyright
