1449-77-0

Usage

Uses

Used in Nuclear Magnetic Resonance (NMR) Spectroscopy:
Formamide-15N is used as a labeling agent for enhancing the detection and analysis of nitrogen-containing compounds in NMR spectroscopy. The presence of the 15N isotope allows for better differentiation and tracking of molecules, providing valuable insights into their structure and dynamics.
Used in Mass Spectrometry:
Formamide-15N is used as a tracer in mass spectrometry to track the movement and transformation of nitrogen-containing compounds. The isotopic signature of 15N aids in the identification and quantification of specific molecules, improving the accuracy and precision of mass spectrometry analysis.
Used in Isotope Dilution Studies:
Formamide-15N is used as a reference material in isotope dilution studies to quantify the amount of specific compounds in a sample. The stable isotopic form allows for accurate measurements and comparisons, ensuring reliable results in various analytical applications.
Used in Research on Nitrogen Metabolism:
Formamide-15N is used as a research tool to examine the origin and pathways of nitrogen metabolism in living organisms. By incorporating the labeled formamide into biological systems, scientists can gain a better understanding of nitrogen cycling and its role in metabolic processes.
Used in Chemical Reaction Studies:
Formamide-15N is used as a reactant in chemical reaction studies to investigate the mechanisms and pathways of nitrogen-containing compounds. The isotopic labeling provides a means to track the progress of reactions and identify intermediates, contributing to a deeper understanding of chemical processes.

Upstream product

• 2067-80-3 urea-15N₂ 
• 64-18-6 formic acid 

Relevant academic research and scientific papers

Stable isotope labeled paclobutrazol-15N3 and synthesis method thereof

The invention discloses stable isotope labeled paclobutrazol-15N3 and a synthesis method thereof. The method includes: taking stable isotope 15N labeled hydrazine sulphate-15N2 and urea-15N2 as stable isotope labeling sources, firstly reacting urea-15N2 w

Photosensitized oxidation of 13C,15N-labeled imidazole derivatives

An efficient synthesis of imidazoles with isotope labeling at different positions of the five-membered ring was developed. We carried out a detailed mechanistic study of the photosensitized oxidation of isotope-labeled imidazole derivatives. A new product, CO2, was observed in the photooxidation of 2-H,N1-H imidazoles, but not in 2-substitituted imidazoles. The carbon of CO2 derives from the 2C of imidazole. As shown by 18O experiments, both oxygen atoms of CO2 originate mainly from one molecule of oxygen. Transient intermediates were detected by low-temperature NMR in the photosensitized oxidation of the isotope-labeled imidazoles. Quantitative analysis of the 13C NMR at different temperatures and times correlates the formation of one intermediate with the loss of another, thus allowing the complete decomposition pathway of the transient intermediates to be established. Singlet oxygen reacts with 4,5-diphenylimidazole via a [4 + 2] cycloaddition to form a 2,5-endoperoxide, which, upon warming, decomposes to a hydroperoxide. The hydroperoxide in one pathway loses water to form an imidazolone 7, which is hydrolyzed to a hydroxyimidazol-2-one 11. In another pathway, the hydroperoxide rearranges to diol 8. The diol rearranges to a carbamate 9 by opening and reclosing the five-membered ring. 9 decomposes to CO2 and benzil diimine. A labile NH in the imidazole is crucial for the decomposition of the initially formed endoperoxide, otherwise the endoperoxide decomposes to regenerate starting material. Many similarities exist between the photooxidations of imidazole and guanosine in organic solvent, suggesting that the two reactions share a similar reaction mechanism with singlet oxygen.

Synthesis of a 13C,15N labeled imidazole and characterization of the 2,5-endoperoxide and its decomposition

A synthesis of 13C, 15N labeled imidazoles was developed which allows the direct detection of a transient 2,5-endoperoxide at -100°C. The endoperoxide was thoroughly characterized by 1H, 13C, and 15N NMR. Upon warming, the endoperoxide decomposes via a series of transient intermediates to 13CO2 and 1,2-diphenyl-ethanediimine. (C) 2000 Elsevier Science Ltd.