146368-16-3

Basic information

• Product Name: CY3 NHS ESTER
• Synonyms: CY3 NHS ESTER;Cy3-SE;Cy3-NHS ester(C35H41N3O10S2);Cy3-SECy3 NHS Ester;Cyanine3 NHS ester;Cyanine 3;Sulfo-Cy3-SE;Sulfo-Cyanine3 NHS ester
• CAS NO: 146368-16-3
• Molecular Formula: C₃₅H₄₁N₃O₁₀S₂
• Mol File: 146368-16-3.mol
• Article Data: 3

Chemical Properties

• Appearance: /
• Storage Temp.: under inert gas (nitrogen or Argon) at 2-8°C
• CAS DataBase Reference: CY3 NHS ESTER(CAS DataBase Reference)
• NIST Chemistry Reference: CY3 NHS ESTER(146368-16-3)
• EPA Substance Registry System: CY3 NHS ESTER(146368-16-3)

Safety Data

• Hazardous Substances Data: 146368-16-3(Hazardous Substances Data)

Usage

Uses

Fluorescent dyes are typically used for imaging applications. Cy3 NHS Ester is a fluorescent dye suitable for labeling proteins and nucleic acids. It exhibits excitation and emission at 555 nm and 570 nm, respectively. Cy3 NHS Ester can be used to label functional nucleic acids to detect tertiary structure folding such as guanine quadruplex.

Upstream product

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Downstream Products

• 1152425-53-0 Cy₃-gabapentin-choline 

Relevant articles and documents

Intracellular pH measurements using perfluorocarbon nanoemulsions

We report the synthesis and formulation of unique perfluorocarbon (PFC) nanoemulsions enabling intracellular pH measurements in living cells via fluorescent microscopy and flow cytometry. These nanoemulsions are formulated to readily enter cells upon coincubation and contain two cyanine-based fluorescent reporters covalently bound to the PFC molecules, specifically Cy3-PFC and CypHer5-PFC conjugates. The spectral and pH-sensing properties of the nanoemulsions were characterized in vitro and showed the unaltered spectral behavior of dyes after formulation. In rat 9L glioma cells loaded with nanoemulsion, the local pH of nanoemulsions was longitudinally quantified using optical microscopy and flow cytometry and displayed a steady decrease in pH to a level of 5.5 over 3 h, indicating rapid uptake of nanoemulsion to acidic compartments. Overall, these reagents enable real-time optical detection of intracellular pH in living cells in response to pharmacological manipulations. Moreover, recent approaches for in vivo cell tracking using magnetic resonance imaging (MRI) employ intracellular PFC nanoemulsion probes to track cells using 19F MRI. However, the intracellular fate of these imaging probes is poorly understood. The pH-sensing nanoemulsions allow the study of the fate of the PFC tracer inside the labeled cell, which is important for understanding the PFC cell loading dynamics, nanoemulsion stability and cell viability over time.

Aryldithioethyloxycarbonyl (Ardec): A new family of amine protecting groups removable under Mild reducing conditions and their applications to peptide synthesis

The development of phenyl-dithioethyloxycarbonyl (Phdec) and 2-pyridyldithioethyloxycarbonyl (Pydec) protecting groups, which are thiollabile urethanes, is described. These new disulfide-based protecting groups were introduced onto the ε-amino group of L-