146954-75-8

Basic information

• Product Name: 5'-O-(4,4-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite
• Synonyms: DMT-2'-F-DU AMIDITE 0.25G, AB, SINGLE;DMT-2'-F-dU Amidite 0.25g, 89, Single;2’-F-rUPhosphoramidite;5'-O-(4,4-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite;5'-O-DMT-2'-F-dU-3'-CEDPA;2'-F-dU Phosphoramidite;2'-Deoxy-2'-fluoro-5'-O-DMT-Uridine-3'-CED PhosphoraMidite;DMT-2′Fluoro-dU Phosphoramidite
• CAS NO: 146954-75-8
• Molecular Formula: C₃₉H₄₆FN₄O₈P
• Molecular Weight: 748.784
• EINECS: 2017-001-1
• Mol File: 146954-75-8.mol
• Article Data: 10

Chemical Properties

• Appearance: white to off-white/
• Storage Temp.: ?20°C
• PKA: 9.39±0.10(Predicted)
• CAS DataBase Reference: 5'-O-(4,4-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite(CAS DataBase Reference)
• NIST Chemistry Reference: 5'-O-(4,4-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite(146954-75-8)
• EPA Substance Registry System: 5'-O-(4,4-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite(146954-75-8)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 146954-75-8(Hazardous Substances Data)

Usage

General Description

The chemical "5'-O-(4,4-Dimethoxytrityl)-2'-deoxy-2'-fluorouridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite" is a phosphoramidite derivative of 2'-deoxy-2'-fluorouridine, a modified nucleoside used in the synthesis of oligonucleotides. The 5'-O-(4,4-Dimethoxytrityl) group is a protective group that helps in the synthesis of the oligonucleotide. The 2'-deoxy-2'-fluorouridine is a modified form of uridine, in which the 2'-hydroxyl group is replaced by a fluorine atom, making it resistant to degradation by nucleases. The 3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite group is a common phosphoramidite protecting group used in DNA and RNA synthesis. Overall, this chemical is essential in the production of modified oligonucleotides for research and therapeutic applications.

Upstream product

• 102691-36-1 N,N,N',N'-tetraisopropyl 2-cyanoethylphosphorodiamidite 
• 146954-74-7 2'-deoxy-2'-fluoro-5'-O-(4,4'-dimethoxytrityl)uridine 
• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 56287-17-3 2'-deoxy-2'-fluorouridine 
• 124482-92-4 2-cyanoethyl-N,N-(diisopropylamino)chlorophosphine 

Relevant articles and documents

Oligonucleotide with protected base

The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve an oligonucleotide production method by a phosphoramidite method. It has been found that the above-mentioned problem can be solved by a particular oligonucleotide comprising a protected base and/or particular oligonucleotide protected by a branched chain-containing aromatic group at 3′-position.

OLIGONUCLEOTIDE WITH PROTECTED BASE

The present invention provides a protected nucleotide for elongation, which can be purified efficiently and in a high yield by a liquid-liquid extraction operation, and can achieve an oligonucleotide production method by a phosphoramidite method. It has been found that the above-mentioned problem can be solved by a particular oligonucleotide comprising a protected base and/or particular oligonucleotide protected by a branched chain-containing aromatic group at 3′-position.

Antisense modulation of polo-like kinase expression

Antisense compounds, compositions and methods are provided for modulating the expression of polo-like kinase. The compositions comprise antisense compounds, particularly antisense oligonucleotides, targeted to nucleic acids encoding polo-like kinase. Methods of using these compounds for modulation of polo-like kinase expression and for treatment of diseases associated with expression of polo-like kinase are provided.