1474020-00-2Relevant articles and documents
Bioorthogonal approach to identify unsuspected drug targets in live cells
Yang, Katherine S.,Budin, Ghyslain,Tassa, Carlos,Kister, Olivier,Weissleder, Ralph
, p. 10593 - 10597 (2013/10/21)
A proteomics method to pull down secondary drug targets from live cells is described. The drug of interest is modified with trans-cyclooctene (TCO) and incubated with live cells. Upon cell lysis, the modified drug bound to the protein is pulled down using magnetic beads decorated with a cleavable tetrazine-modified linker. Samples are then run on an SDS-PAGE gel and isolated bands are submitted for mass spectrometry analysis to identify drug targets. Copyright