14843-73-3

Basic information

• Product Name: 2'-O-fucosyllactose
• CAS NO: 14843-73-3
• Molecular Weight: 488.443
• Mol File: 14843-73-3.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: 2'-O-fucosyllactose(CAS DataBase Reference)
• NIST Chemistry Reference: 2'-O-fucosyllactose(14843-73-3)
• EPA Substance Registry System: 2'-O-fucosyllactose(14843-73-3)

Safety Data

• Hazardous Substances Data: 14843-73-3(Hazardous Substances Data)

Upstream product

• 991-43-5 guanosine 5'-diphospho-D-mannose 

Downstream Products

• 1363444-66-9 C₃₃H₅₂N₆O₂₁ 
• 1363444-62-5 1-p-nitrobenzyl-(2-fucosyl)-lactose 

Relevant articles and documents

Synthesis of the milk oligosaccharide 2′-fucosyllactose using recombinant bacterial enzymes

The enzymatic synthesis of GDP-β-L-fucose and its enzymatic transfer reaction using recombinant enzymes from bacterial sources was examined. The GDP-D-mannose 4,6-dehydratase and the GDP-4-keto-6-deoxy-D-mannose 3,5-epimerase-4-reductase from Escherichia coli K-12, respectively, were used to catalyse the conversion of GDP-α-D-mannose to GDP-β-L-fucose with 78% yield. For the transfer of the L-fucose to an acceptor, we cloned and overproduced the α-(1 → 2)-fucosyltransferase (FucT2) protein from Helicobacter pylori. We were able to synthesise 2′-fucosyllactose using the overproduced FucT2 enzyme, enzymatically synthesised GDP-L-fucose and lactose. The isolation of 2′-fucosyllactose was accomplished by anion-exchange chromatography and gel filtration to give 65% yield.