148892-91-5

Basic information

• Product Name: 5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM
• Synonyms: 5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM;guanosine 5'-[β,γ-imido]triphosphate trisodium salt;β:γ-imidoguanosine 5'-triphosphate trisodium salt;5μ-Guanylyl-imidodiphosphate hydrate trisodium salt, GMP-PNP, Gpp(NH)p, β:γ-Imidoguanosine 5μ-triphosphate hydrate trisodium salt;Guanosine 5μ-[β,γ-imido]triphosphate hydrate trisodium salt;5′-Guanylyl-imidodiphosphate trisodium salt hydrate;Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate;β:γ-Imidoguanosine 5′-triphosphate trisodium salt hydrate
• CAS NO: 148892-91-5
• Molecular Formula: C10H13N6Na3O10P2
• Molecular Weight: 508.161252
• Mol File: 148892-91-5.mol
• Article Data: 0

Chemical Properties

• Appearance: white/powder
• Storage Temp.: −70°C
• Solubility: H2O: 50 mg/mL
• CAS DataBase Reference: 5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM(CAS DataBase Reference)
• NIST Chemistry Reference: 5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM(148892-91-5)
• EPA Substance Registry System: 5'-GUANYLYLIMIDODIPHOSPHATE TRISODIUM(148892-91-5)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 26-36/37
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 148892-91-5(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 148892-91-5 differently. You can refer to the following data:
1. Non-hydrolyzable analog of GTP; the preferred GTP analog to activate ADP-ribosylation factor.
2. Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate has been used: to study K-RAS interactions with the membrane, for the crystallization of Mycobacterium tuberculosis FtsZ, as a translation initiation inhibitor for the analysis of the 48S pre-initiation complex.

General Description

Guanosine 5′-[β,γ-imido]triphosphate (GMP-PNP, Gpp(NH)p) is a non-hydrolyzable analog of GTP that binds and irreversibly activates G proteins. Gpp(NH)p is used in a variety of applications that involve GTP binding, including GTP-activation, GTP-inhibition, GTP transport, GTP hydrolysis, and GTP structure stabilization. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, guanosine 5′-[β,γ-imido]triphosphate is often used in studies of protein synthesis. Gpp(NH)p is used to activate ADP-ribosylation factors and to modulate G proteins involved in cell signaling, protein synthesis and other metabolic processes.

Biochem/physiol Actions

Guanosine 5′-[?,γ-imido]triphosphate binds and irreversibly activates G proteins. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, this non-hydrolyzable GTP analog is often used in studies of protein synthesis.