153162-70-0Relevant articles and documents
RNA G-Quadruplexes in Kirsten Ras (KRAS) Oncogene as Targets for Small Molecules Inhibiting Translation
Miglietta, Giulia,Cogoi, Susanna,Marinello, Jessica,Capranico, Giovanni,Tikhomirov, Alexander S.,Shchekotikhin, Andrey,Xodo, Luigi E.
, p. 9448 - 9461 (2017)
The human KRAS transcript contains a G-rich 5′-UTR sequence (77% GC) harboring several G4 motifs capable to form stable RNA G-quadruplex (RG4) structures that can serve as targets for small molecules. A biotin-streptavidin pull-down assay showed that 4,11-bis(2-aminoethylamino)anthra[2,3-b]furan-5,10-dione (2a) binds to RG4s in the KRAS transcript under low-abundance cellular conditions. Dual-luciferase assays demonstrated that 2a and its analogue 4,11-bis(2-aminoethylamino)anthra[2,3-b]thiophene-5,10-dione (2b) repress translation in a dose-dependent manner. The effect of the G4-ligands on Panc-1 cancer cells has also been examined. Both 2a and 2b efficiently penetrate the cells, suppressing protein p21KRAS to a dramatic reduction of cell growth and colony formation. In summary, we report a strategy to suppress the KRAS oncogene in pancreatic cancer cells by means of small molecules binding to RG4s in the 5′-UTR of mRNA.
Photocleavable ligands for protein decoration of DNA nanostructures
Brglez, Josipa,Ahmed, Ishtiaq,Niemeyer, Christof M.
, p. 5102 - 5104 (2015)
This work describes the synthesis of amino-reactive, photocleavable hapten-modifiers and their application as affinity tags for DNA nanostructures. In particular, N-hydroxysuccinimide-activated linkers containing an α-methyl-nitroveratryl-butyric acid group and carboxyfluorescein or biotin were synthesized and coupled to alkyl-amino-modified DNA oligonucleotides. The resulting conjugates were then incorporated into DNA origami nanostructures. As demonstrated by electrophoresis and AFM imaging, the functionalized nanostructures were capable to bind cognate proteins which could then be cleaved-off by irradiation. Owing to its modularity, this approach to control protein binding should be useful for a wide variety of functional DNA nanostructures.
PYRIDAZINONES AS PARP7 INHIBITORS
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Page/Page column 162-163; 165, (2021/05/07)
The present invention relates to pyridazinones and related compounds which are inhibitors of PARP7 and are useful in the treatment of cancer.
In Vitro and Cellular Probes to Study PARP Enzyme Target Engagement
Blackwell, Danielle J.,Church, W. David,Desai, Hetvi J.,Keilhack, Heike,Kuntz, Kevin W.,Lu, Alvin Z.,Majer, Christina R.,Niepel, Mario,Perl, Nicholas R.,Ren, Yue,Santospago, Andrew G.,Schenkel, Laurie B.,Swinger, Kerren K.,Vasbinder, Melissa M.,Wigle, Tim J.
supporting information, p. 877 - 887 (2020/07/16)
Poly(ADP-ribose) polymerase (PARP) enzymes use nicotinamide adenine dinucleotide (NAD+) to modify up to seven different amino acids with a single mono(ADP-ribose) unit (MARylation deposited by PARP monoenzymes) or branched poly(ADP-ribose) polymers (PARylation deposited by PARP polyenzymes). To enable the development of tool compounds for PARP monoenzymes and polyenzymes, we have developed active site probes for use in in vitro and cellular biophysical assays to characterize active site-directed inhibitors that compete for NAD+ binding. These assays are agnostic of the protein substrate for each PARP, overcoming a general lack of knowledge around the substrates for these enzymes. The in vitro assays use less enzyme than previously described activity assays, enabling discrimination of inhibitor potencies in the single-digit nanomolar range, and the cell-based assays can differentiate compounds with sub-nanomolar potencies and measure inhibitor residence time in live cells. Wigle et al. describe a versatile set of NAD+-competitive probes for PARP enzymes that are used to build high-throughput in vitro and cellular biophysical assays that enable inhibitor screening and determination of residence time.
