58-85-5Relevant academic research and scientific papers
SYSTEMS AND METHODS FOR DETECTION OF TARGET ANALYTES USING SELECTIVELY CLEAVABLE BONDS
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, (2021/09/10)
The invention described herein is directed to methods of isolation and detection of target analytes in a sample. The target analytes are coupled to analyte detection particles which comprise base particles having labels and affinity agents coupled thereto by linker arms. The linker arms form bonds with the labels and target analytes and are cleavable under different label and affinity cleavable conditions. Systems and methods for preparing and using the analyte detection particles are also disclosed.
METHOD FOR PRODUCING BIOTIN
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Paragraph 0067-0083, (2021/02/25)
To provide a production method for obtaining high-purity biotin at good yields.SOLUTION: A method for producing biotin includes a process in which a composition comprising biotin represented by the formula (I) is brought into contact with hydrosulfite salt, before the biotin is extracted.SELECTED DRAWING: None
Solution Dynamics of Hybrid Anderson-Evans Polyoxometalates
Salazar Marcano, David E.,Lentink, Sarah,Moussawi, Mhamad A.,Parac-Vogt, Tatjana N.
supporting information, p. 10215 - 10226 (2021/05/31)
Understanding the stability and speciation of metal-oxo clusters in solution is essential for many of their applications in different areas. In particular, hybrid organic-inorganic polyoxometalates (HPOMs) have been attracting increasing attention as they combine the complementary properties of organic ligands and metal-oxygen nanoclusters. Nevertheless, the speciation and solution behavior of HPOMs have been scarcely investigated. Hence, in this work, a series of HPOMs based on the archetypical Anderson-Evans structure, δ-[MnMo6O18{(OCH2)3C-R}2]3-, with different functional groups (R = -NH2, -CH3, -NHCOCH2Cl, -NCH(2-C5H4N) {pyridine; -Pyr}, and -NHCOC9H15N2OS {biotin; -Biot}) and countercations (tetrabutylammonium {TBA}, Li, Na, and K) were synthesized, and their solution behavior was studied in detail. In aqueous solutions, decomposition of HPOMs into the free organic ligand, [MoO4]2-, and free Mn3+ was observed over time and was shown to be highly dependent on the pH, temperature, and nature of the ligand functional group but largely independent of ionic strength or the nature of the countercation. Furthermore, hydrolysis of the amide and imine bonds often present in postfunctionalized HPOMs was also observed. Hence, HPOMs were shown to exhibit highly dynamic behavior in solution, which needs to be carefully considered when designing HPOMs, particularly for biological applications.
Selective modification of sulfamidate-containing peptides
Busto, Jesús H.,Jiménez-Osés, Gonzalo,Mazo, Nuria,Navo, Claudio D.,Peregrina, Jesús M.
supporting information, p. 6265 - 6275 (2020/09/07)
Hybrid peptides whose N-terminal residues are activated in the form of α-methylisoserine-derived cyclic sulfamidates exhibit rich reactivity as electrophiles, allowing site- and stereoselective modifications at different backbone and side chain positions.
Synthesis method of d-biotin
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, (2019/04/04)
The invention discloses a synthesis method of d-biotin. The synthesis method comprises the following steps: taking cysteine hydrochloride, benzaldehyde and benzyl isocyanate as starting materials, andsubjecting the starting materials to condensation, cyclization, reduction, condensation, oxidation, reduction, cyclization, elimination, catalytic hydrogenation and debenzylation sequentially to obtain the d-biotin. The method has the advantages that the raw materials are cheap and easy to obtain, the safety is good, the reaction conditions are mild and easy to control, the production cost is lowered, green and environmental protection is achieved, the reaction yield is high, separation is easy, and the purity of the obtained d-biotin is high.
Method for catalytic synthesis of biotin by using high-recycling-activity palladium-carbon catalyst
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Paragraph 0019; 0020-0057, (2019/04/10)
The invention discloses a method for catalytic synthesis of biotin by using a high-recycling-activity palladium-carbon catalyst. The method comprises the following steps: palladium-carbon is taken asa catalyst to catalyze synthesis of biotin by hydrogenation of a biotin intermediate cis-2-oxo-1,3-dibenzyl-4-(4-carboxybutyl-1-ene)hexahydro-1H-thieno[3,4-d]imidazole, wherein activated carbon is taken as a carrier of the catalyst, the carrier is activated by hydrogen peroxide after being subjected to high-temperature treatment in an ammonia atmosphere, and then the pretreated activated carbon isobtained; the soluble palladium compound is dissolved in water, a nitrogen-containing compound is added, and reflux stirring is carried out to obtain a palladium precursor solution; and the pretreated activated carbon is pulped by using alcohol water, then a palladium precursor solution is added, an alkali is added for controlling the pH value of the system to be 6-12, and then reduction is carried out by a reducing agent to obtain the palladium-carbon catalyst. Compared with the prior art, the method disclosed by the invention has the advantages that activity of the catalyst is high, performance of the catalyst is stable, the number of recycling times in biotin synthesis can be greatly increased, and production cost can be reduced.
A preparation method of d - biotin (by machine translation)
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Paragraph 0037; 0038; 0039; 0040; 0041; 0042; 0043-0060, (2019/05/04)
The invention belongs to the field of organic synthesis, in particular relates to a preparation method of d - biotin, the method comprises the following steps: the double-benzyl biotin with three boron halide and organic solution, in the absence of the organic solvent in the water environment, under the protection of inert gas, through the one-step reaction to remove the double-benzyl, get d - biotin. The present invention provides a simple and convenient, safe, high yield, high purity of methylsulphonyl preparation method of d - biotin, is suitable for industrial production. (by machine translation)
A dibenzyl biotin by d - biotin method of preparation
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Paragraph 0033-0038, (2019/02/26)
The invention discloses a method for preparing d-biotin from dibenzyl biotin. The method comprises the following steps: 1, adding an inorganic acid and zinc powder to an aqueous solution of dibenzyl biotin; and 2, reacting at 40-70DEG C, and post-processing after the raction ends to obtain d-biotin. The d-biotin is prepared through removing benzyl groups by using the zinc powder/inorganic acid system under mild conditions, so the method has the advantages of simple operation and easy industrialization; and the purity of the obtained d-biotin is greater than 99.5%, and the mole yield of the obtained d-biotin is greater than 90%, so compared with the prior art, the method has substantial progress.
Method for Producing Intermediate of Biotin and Method for Producing Biotin
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, (2020/01/08)
In the method, a trione compound represented by the following formula (1) is (i) reduced by NaAlH2(OCH2CH2OCH3)2 and subsequently further reduced by a metal borohydride salt, or (ii) reduced by calcium borohydride, thereby producing an amide alcohol compound represented by the following formula (3) (wherein, R1 and R2 may be the same or different and each represents a hydrogen atom or a protecting group of an ureylene group; R4 represents an alkyl group, an aralkyl group, or an aryl group; and each of R5, R6, and R7 represents a hydrogen atom, an alkyl group, an alkoxy group, or a halogen atom).
Selective Modification of Ribosomally Synthesized and Post-Translationally Modified Peptides (RiPPs) through Diels–Alder Cycloadditions on Dehydroalanine Residues
de Vries, Reinder H.,Viel, Jakob H.,Oudshoorn, Ruben,Kuipers, Oscar P.,Roelfes, Gerard
supporting information, p. 12698 - 12702 (2019/09/12)
We report the late-stage chemical modification of ribosomally synthesized and post-translationally modified peptides (RIPPs) by Diels–Alder cycloadditions to naturally occurring dehydroalanines. The tail region of the thiopeptide thiostrepton could be modified selectively and efficiently under microwave heating and transition-metal-free conditions. The Diels–Alder adducts were isolated and the different site- and endo/exo isomers were identified by 1D/2D 1H NMR. Via efficient modification of the thiopeptide nosiheptide and the lanthipeptide nisin Z the generality of the method was established. Minimum inhibitory concentration (MIC) assays of the purified thiostrepton Diels–Alder products against thiostrepton-susceptible strains displayed high activities comparable to that of native thiostrepton. These Diels–Alder products were also subjected successfully to inverse-electron-demand Diels–Alder reactions with a variety of functionalized tetrazines, demonstrating the utility of this method for labeling of RiPPs.
