153248-58-9 Usage
General Description
N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a chemical compound used in the synthesis of RNA molecules. It contains acetyl, dimethoxytrityl, tert-butyl, dimethylsilyl, and cyanoethyl groups, as well as a phosphoramidite functional group. These groups allow for the efficient and precise assembly of RNA molecules using solid-phase synthesis techniques. The chemical is commonly used in research and pharmaceutical development for the creation of custom RNA sequences for various applications, including gene expression studies, therapeutic development, and biochemical research.
Check Digit Verification of cas no
The CAS Registry Mumber 153248-58-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,5,3,2,4 and 8 respectively; the second part has 2 digits, 5 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 153248-58:
(8*1)+(7*5)+(6*3)+(5*2)+(4*4)+(3*8)+(2*5)+(1*8)=129
129 % 10 = 9
So 153248-58-9 is a valid CAS Registry Number.
153248-58-9Relevant articles and documents
Fingerprints of Modified RNA Bases from Deep Sequencing Profiles
Kietrys, Anna M.,Velema, Willem A.,Kool, Eric T.
, p. 17074 - 17081 (2017)
Posttranscriptional modifications of RNA bases are not only found in many noncoding RNAs but have also recently been identified in coding (messenger) RNAs as well. They require complex and laborious methods to locate, and many still lack methods for localized detection. Here we test the ability of next-generation sequencing (NGS) to detect and distinguish between ten modified bases in synthetic RNAs. We compare ultradeep sequencing patterns of modified bases, including miscoding, insertions and deletions (indels), and truncations, to unmodified bases in the same contexts. The data show widely varied responses to modification, ranging from no response, to high levels of mutations, insertions, deletions, and truncations. The patterns are distinct for several of the modifications, and suggest the future use of ultradeep sequencing as a fingerprinting strategy for locating and identifying modifications in cellular RNAs.
