153248-58-9 Usage
Description
N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is a complex chemical compound specifically designed for the synthesis of RNA molecules. It incorporates a variety of functional groups, including acetyl, dimethoxytrityl, tert-butyl, dimethylsilyl, and cyanoethyl, along with a phosphoramidite functional group. These components enable the efficient and accurate assembly of RNA sequences through solid-phase synthesis methods, making it a valuable tool in the fields of research and pharmaceutical development.
Uses
Used in Research and Pharmaceutical Development:
N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is used as a key component in the synthesis of custom RNA sequences for various applications. It is particularly valuable for gene expression studies, where precise control over RNA sequences is crucial for understanding gene function and regulation.
Used in Biochemical Research:
In biochemical research, this compound serves as a building block for creating RNA molecules with specific properties, allowing scientists to investigate the structure, function, and interactions of RNA at a molecular level. Its use in this context contributes to a deeper understanding of RNA biology and its role in cellular processes.
Used in Therapeutic Development:
N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is also utilized in the development of RNA-based therapeutics. Its ability to facilitate the synthesis of RNA molecules with specific sequences makes it an essential tool in creating potential treatments for a range of diseases, including genetic disorders and viral infections.
Used in the Synthesis of Modified Nucleosides:
N-Acetyl-5'-O-(4,4-dimethoxytrityl)-2'-O-[(tert-butyl)dimethylsilyl]-6'-O-methylguanosine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite is used as a precursor in the synthesis of modified nucleosides, which are important for the development of novel RNA molecules with enhanced stability, specificity, and functionality. These modified nucleosides can be incorporated into RNA sequences to improve their performance in various applications, such as RNA interference, antisense therapy, and aptamer development.
Check Digit Verification of cas no
The CAS Registry Mumber 153248-58-9 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,5,3,2,4 and 8 respectively; the second part has 2 digits, 5 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 153248-58:
(8*1)+(7*5)+(6*3)+(5*2)+(4*4)+(3*8)+(2*5)+(1*8)=129
129 % 10 = 9
So 153248-58-9 is a valid CAS Registry Number.
153248-58-9Relevant articles and documents
Fingerprints of Modified RNA Bases from Deep Sequencing Profiles
Kietrys, Anna M.,Velema, Willem A.,Kool, Eric T.
, p. 17074 - 17081 (2017)
Posttranscriptional modifications of RNA bases are not only found in many noncoding RNAs but have also recently been identified in coding (messenger) RNAs as well. They require complex and laborious methods to locate, and many still lack methods for localized detection. Here we test the ability of next-generation sequencing (NGS) to detect and distinguish between ten modified bases in synthetic RNAs. We compare ultradeep sequencing patterns of modified bases, including miscoding, insertions and deletions (indels), and truncations, to unmodified bases in the same contexts. The data show widely varied responses to modification, ranging from no response, to high levels of mutations, insertions, deletions, and truncations. The patterns are distinct for several of the modifications, and suggest the future use of ultradeep sequencing as a fingerprinting strategy for locating and identifying modifications in cellular RNAs.
