16009-13-5 Usage
Chemical Properties
Dark purple crystalline powder
Uses
Different sources of media describe the Uses of 16009-13-5 differently. You can refer to the following data:
1. Identification of blood stains, biochemical research, complexing agent.
2. Hemin is used in cellular protection and control mechanism. It stimulates the synthesis of globulin. It is also used to study the bacterial strains and growth conditions, in the short-term colony assays and cell transfections. Further, it is used to treat the symptoms of various porphyrias. In addition, it is used in the preparation of agar medium for the bacteria culture.
3. Hemin is used for the following applications:To study bacterial strains and growth conditionsUsed in short-term colony assays (the culture medium consists of hemin along with other components)Cell transfectionsIt may be used in β-hematin formation assay for analyzing the inhibitory activity of MMV (Medicines for Malaria Venture) Malaria Box compounds against the formation of β-hematin.
General Description
Heme is a small molecule present either free or bound to hemoglobin in the bloodstream of mammals. This is an alternative source of iron within the host that contains a porphyrin ring containing a Fe2+ ion called hemin.
Biochem/physiol Actions
Hemin, the oxidized version of heme, is an iron-containing prosthetic group for a diverse group of proteins. Free heme, which can be released from hemoglobin following hemolysis, is pro-inflammatory and contributes to iron-derived reactive oxygen species. Free hemin levels may be upregulated in various pathological conditions and can contribute to various inflammatory conditions including vascular disorders, renal failure, and immune-mediated disorders.Hemin may be used to study the expression and represson of heme oxygenase 1 (HO-1) as well as the activity of HO-1. Hematin is the "X factor" required to grown Haemophilus influenzae.
Purification Methods
Hemin is purified by recrystallisation from AcOH. Also, hemin (5g) is shaken in pyridine (25mL) till it dissolves, then CHCl3 (40mL) is added, the container is stoppered and shaken for 5minutes (releasing the stopper occasionally). The solution is filtered under slight suction, and the flask and filter are washed with a little CHCl3 (15mL). During this period, AcOH (300mL) is heated to boiling, and saturated aqueous NaCl (5mL) and conc HCl (4mL) are added. The CHCl3 filtrate is poured in a steady stream, with stirring, into the hot AcOH mixture and set aside for 12hours. The crystals are filtered off, washed with 50% aqueous AcOH (50mL), H2O (100mL), EtOH (25mL), Et2O and dried in air. [Fischer Org Synth Coll Vol III 442 1955, Beilstein 26 III/IV 3048.]
Check Digit Verification of cas no
The CAS Registry Mumber 16009-13-5 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,6,0,0 and 9 respectively; the second part has 2 digits, 1 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 16009-13:
(7*1)+(6*6)+(5*0)+(4*0)+(3*9)+(2*1)+(1*3)=75
75 % 10 = 5
So 16009-13-5 is a valid CAS Registry Number.
InChI:InChI=1/C34H34N4O4.ClH.Fe/c1-7-21-17(3)25-13-26-19(5)23(9-11-33(39)40)31(37-26)16-32-24(10-12-34(41)42)20(6)28(38-32)15-30-22(8-2)18(4)27(36-30)14-29(21)35-25;;/h7-8,13-16H,1-2,9-12H2,3-6H3,(H4,35,36,37,38,39,40,41,42);1H;/q;;+3/p-3/b25-13-,26-13-,27-14-,28-15-,29-14-,30-15-,31-16-,32-16-;;/rC34H32ClFeN4O4/c1-7-21-17(3)26-14-31-22(8-2)19(5)29-13-25-18(4)23(9-11-33(41)42)28(38-25)16-32-24(10-12-34(43)44)20(6)30(15-27(21)37-26)40(32)36(35)39(29)31/h7-8,13-16H,1-2,9-12H2,3-6H3,(H,41,42)(H,43,44)/b25-13-,26-14-,27-15-,28-16-,29-13-,30-15-,31-14-,32-16-
16009-13-5Relevant articles and documents
Impact of metal ions in porphyrin-based applied materials for visible-light photocatalysis: Key information from ultrafast electronic spectroscopy
Kar, Prasenjit,Sardar, Samim,Alarousu, Erkki,Sun, Jingya,Seddigi, Zaki S.,Ahmed, Saleh A.,Danish, Ekram Y.,Mohammed, Omar F.,Pal, Samir Kumar
, p. 10475 - 10483 (2014/08/18)
ProtoporphyrinIX-zinc oxide (PP-ZnO) nanohybrids have been synthesized for applications in photocatalytic devices. High-resolution transmission electron microscopy (HRTEM), X-ray diffraction (XRD), and steady-state infrared, absorption, and emission spect
