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NITRO BLUE TETRAZOLIUM FORMAZAN, often referred to as NBT, is a chemical compound widely utilized in biological and biochemical assays for the detection of specific enzymes and metabolic activities. It is particularly known for its ability to form an insoluble blue formazan precipitate upon reduction by enzymes such as dehydrogenases, offering a visual indication of enzyme activity. NBT is especially valuable in identifying superoxide radicals generated by cells during oxidative stress, making it an essential tool in the study of cellular processes like respiration and photosynthesis. Its capacity to produce a visible color change renders NBT a significant reagent in biochemistry and molecular biology.

16325-01-2

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16325-01-2 Usage

Uses

Used in Biochemical Assays:
NITRO BLUE TETRAZOLIUM FORMAZAN is used as a detection agent for identifying the presence of certain enzymes and their activities. It serves as a visual indicator when these enzymes reduce NBT, leading to the formation of a blue formazan precipitate.
Used in Cellular Process Studies:
In the field of cellular process research, NITRO BLUE TETRAZOLIUM FORMAZAN is used as a tool for studying respiration and photosynthesis. Its ability to detect superoxide radicals makes it instrumental in understanding the role of these radicals in cellular metabolism and stress responses.
Used in Oxidative Stress Research:
NITRO BLUE TETRAZOLIUM FORMAZAN is used as a reagent in oxidative stress research to detect the production of superoxide radicals by cells. This helps in evaluating the cellular response to various stressors and the mechanisms of oxidative damage.
Used in Biochemistry and Molecular Biology:
In the application industry of biochemistry and molecular biology, NITRO BLUE TETRAZOLIUM FORMAZAN is used as a valuable reagent for its capacity to produce a visible color change upon enzyme reduction. This feature aids researchers in visualizing and quantifying enzyme activity and related metabolic processes.

Check Digit Verification of cas no

The CAS Registry Mumber 16325-01-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,6,3,2 and 5 respectively; the second part has 2 digits, 0 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 16325-01:
(7*1)+(6*6)+(5*3)+(4*2)+(3*5)+(2*0)+(1*1)=82
82 % 10 = 2
So 16325-01-2 is a valid CAS Registry Number.
InChI:InChI=1/C40H32N10O6/c1-55-37-25-29(13-23-35(37)43-47-39(27-9-5-3-6-10-27)45-41-31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)44-48-40(28-11-7-4-8-12-28)46-42-32-17-21-34(22-18-32)50(53)54/h3-26,41-42H,1-2H3/b45-39+,46-40+,47-43+,48-44+

16325-01-2SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 10, 2017

Revision Date: Aug 10, 2017

1.Identification

1.1 GHS Product identifier

Product name NITRO BLUE TETRAZOLIUM FORMAZAN

1.2 Other means of identification

Product number -
Other names 5,5'-Bis(4-nitrophenyl)-3,3'-diphenyl-1,1'-[(3,3'-dimethoxy)-4,4'-bisphenylene]diformazan

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:16325-01-2 SDS

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