Nitro blue tetrazolium (NBT) is a chemical compound widely used in biological and medical research as a colorimetric indicator. It is a yellow, water-soluble dye that, upon reduction, forms a blue, water-insoluble formazan product. NBT is commonly employed in enzyme-linked immunosorbent assays (ELISA) and other diagnostic tests to detect the presence of enzymes, such as catalase and peroxidase, by monitoring the color change from yellow to blue. This color change occurs when the enzyme reduces the NBT, indicating the enzyme's activity and presence in the sample. Additionally, NBT is used in the nitro blue tetrazolium reduction test (NBT test) to assess the respiratory burst activity of neutrophils, a key component of the immune response, by measuring the ability of these cells to reduce NBT to formazan. Overall, nitro blue tetrazolium plays a significant role in various scientific applications, providing a visual and quantitative assessment of enzyme activity and immune cell function.
The CAS Registry Mumber 7695-60-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 7,6,9 and 5 respectively; the second part has 2 digits, 6 and 0 respectively. Calculate Digit Verification of CAS Registry Number 7695-60: (6*7)+(5*6)+(4*9)+(3*5)+(2*6)+(1*0)=135 135 % 10 = 5 So 7695-60-5 is a valid CAS Registry Number.