168294-75-5Relevant academic research and scientific papers
An improvede synthesis of 1-(2-deoxy-β-D-erythro-pentofur-anosyl)quinazoline-2,4(3H)-dione and its incorporation into G-rich triple helix forming oligonucleotides
Bhattacharya,Chari,Durland,Revankar
, p. 45 - 63 (1995)
A convenient synthesis of 1-(2-deoxy-β-D-erythro-pentofuranosyl)quinazoline-2,4(3H)-dione (6) has been accomplished. The structural conformation of (6) was derived by 2D NMR, COSY and NOESY experiments. Nucleoside (6) was incorporated into G-rich triplex forming oligonucleotides (TFOs) by solid-support, phosphoramidite method. The triplex forming capabilities of modified TFOs (S2, S3 and S4) has been evaluated in antiparallel motif with a target duplex (duplex-31) 5'd(GTCACTGGCCCTTCCTCCTTCCCGGTCTCAG)3'-5'd(CAGTGACCGGGAAGGAGGA AGGGCCAG AGTC)3' (D1) at pH 7.6. The parallel triplex formation of a shorter TFO (S6) containing Q has also been studied with a target duplex-11 (D2) at pH 5.0.
