171284-50-7

Upstream product

• 124-40-3 dimethyl amine 
• 171284-49-4 2-fluoro-O⁶-<2-(4-nitrophenyl)ethyl>inosine 
• 91592-85-7 (2R,3R,4S,5R)-2-(2-amino-6-(4-nitrophenethoxy)-9H-purin-9-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol 
• 171284-47-2 2′,3′,5′-tri-O-acetyl-O⁶-[2-(4-nitrophenyl)ethyl]guanosine 
• 171284-48-3 2',3',5'-tris-O-acetyl-2-fluoro-O⁶-<2-(4-nitrophenyl)ethyl>inosine 

Downstream Products

• 661471-77-8 5'-O-(4,4'-dimethoxytrityl)-N²,N²-dimethyl-O⁶-[2-(4-nitrophenyl)ethyl]guanosine 

Relevant academic research and scientific papers

The Synthesis of 2′-O-[(Triisopropylsilyl)oxy] methyl (TOM) Phosphoramidites of Methylated Ribonucleosides (m1G, m2G, m22G, m1I, m3U, m4C, m6A, m62A) for Use in Automated RNA Solid-Phase Synthesis

The straightforward synthesis of eight methylated ribonucleoside phosphoramidites is described. These building blocks allow for incorporation of the naturally occuring nucleosides 1-methylguanosine (m1G), N 2-methylguanosine (m2G), N2N 2-dimethylguanosine (m22G), 1-methylinosine (m1I), 3-methyluridine (m3U), N4- methylcytidine (m4C), N6-methyladenosine (m6A), and N6,N6-dimethyladenosine (m62A) into oligoribonucleotides by automated RNA solid-phase synthesis. In all cases, the ribose 2′-hydroxyl group of the building blocks is masked by the recently introduced [(triisopropylsilyl)oxy]methyl (TOM) group.

A convenient method for the preparation of N2,N2-dimethylguanosine

The preparation of N2,N2-dimethylguanosine is described. The use of the 2-(p-nitrophenyl)ethyl group instead of the benzyl protecting group for the O6 position of the guanine ring resulted in better yields and shorter protocols.