1738-97-2Relevant academic research and scientific papers
THE USE OF PENICILLIN ACYLASE FOR SELECTIVE N-TERMINAL DEPROTECTION IN PEPTIDE SYNTHESIS
Waldmann, Herbert
, p. 1131 - 1134 (2007/10/02)
Penicillin acylase from E. coli (EC 3.5.1.11) accepts a broad range of N-phenylacetyl-dipeptide esters as substrates.The enzyme hydrolyses the N-terminal protecting group selectively at room temp. and pH=8.1 without affecting the peptide- or the ester-bonds.Alternatively methyl-, benzyl-, tert-butyl and allyl esters can be cleaved chemically leaving the phenylacetamido moiety intact.
