17695-46-4

Basic information

• Product Name: 4-METHYLUMBELLIFERYL BUTYRATE
• Synonyms: 4-methyl-2-oxo-2H-1-benzopyran-7-yl butyrate;4-METHYLUMBELLIFERYL BUTYRATE, FOR FLUOR ESCENCE;forfluorescence,95+%;mu-bu;4-METHYLUMBELLIFERYL BUTYRATE FOR FLUORESCENCE 95+%;Butyric acid 2-oxo-4-methyl-2H-1-benzopyran-7-yl ester;Butyric acid 4-methyl-2-oxo-2H-1-benzopyran-7-yl ester;METHYLUMBELLIFERYL BUTYRATE, 4-
• CAS NO: 17695-46-4
• Molecular Formula: C₁₄H₁₄O₄
• Molecular Weight: 246.26
• EINECS: 241-694-7
• Product Categories: Substrates
• Mol File: 17695-46-4.mol
• Article Data: 5

Chemical Properties

• Melting Point: 90-92 °C(lit.)
• Boiling Point: 393.4 °C at 760 mmHg
• Flash Point: 199.8 °C
• Appearance: /
• Density: 1.197 g/cm³
• Vapor Pressure: 2.13E-06mmHg at 25°C
• Refractive Index: 1.545
• Storage Temp.: −20°C
• Solubility: DMF: soluble
• BRN: 227973
• CAS DataBase Reference: 4-METHYLUMBELLIFERYL BUTYRATE(CAS DataBase Reference)
• NIST Chemistry Reference: 4-METHYLUMBELLIFERYL BUTYRATE(17695-46-4)
• EPA Substance Registry System: 4-METHYLUMBELLIFERYL BUTYRATE(17695-46-4)

Safety Data

• Hazard Codes: T+
• Statements: 26/27/28
• Safety Statements: 24/25
• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 17695-46-4(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 17695-46-4 differently. You can refer to the following data:
1. Fluorogenic substrate for butyrate esterase.
2. 4-Methylumbelliferyl Butyrate is suitable to use as a fluorogenic substrate for esterases/lipases, such as butyrate esterase.

Definition

ChEBI: A member of the class of coumarins that is 4-methylumbelliferone in which the hydroxyl hydrogen is replaced by a butyryl group.

InChI:InChI=1/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3

Upstream product

• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 141-75-3 butyryl chloride 
• 106-31-0 butanoic acid anhydride 

Downstream Products

• 90-33-5 7-hydroxy-4-methyl-chromen-2-one 
• 107-92-6 butyric acid 
• 105-54-4 butanoic acid ethyl ester 
• 623-42-7 butanoic acid methyl ester 
• 90104-80-6 8-butyryl-7-hydroxy-4-methyl-coumarin 
• 35031-57-3 1-(2,4-dimethylphenyl)butan-1-one 

Relevant articles and documents

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METHOD FOR DETECTION AND/OR ASSAY OF LOVASTATIN ESTERASE WITH USE OF FLUOROGENIC/CHROMOGENIC REAGENT, LOVASTATIN ESTERASE ISOLATED AND/OR PURIFIED BY THIS METHOD, ASSEMBLY FOR DETECTION AND/OR ASSAY AND USE OF FLUOROGENIC/CHROMOGENIC REAGENT FOR DETECTION AND/OR ASSAY OF LOVASTATIN ESTERASE

The invention relates to a method for detection and/or assay of the lovastatin esterase enzymatic activity, with use of the fiuorogenic/chromogenic reagent, comprising use of the fiuorogenic/chromogenic reagent of formula II wherein R represents hydrogen or C1-C6-alkyl, R' represents one or two substituents of the ring A, R" represents one or two or three substituents of the ring B. According to a method, the reagent is added to a material of enzymatic activity and the hydrolysis reaction under enzyme action is carried out, followed by detection and/or assay of the liberated coumarin compound of formula III. Also the invention relates to lovastatin esterase isolated and/or purified using this method, an assembly for detection and/or assay of the lovastatin esterase enzymatic activity, an use of the fiuorogenic/chromogenic reagent of formula II for detection and/or assay of the lovastatin esterase enzymatic activity.

A high-throughput, low-volume enzyme assay on solid support

A high-throughput enzyme assay is described that uses 1 μL or less of enzyme solution for each test Enzyme solutions are deposited by robotic handling in a throughput of over 1000 tests/h on the surface of silica gel plates that have been preimpregnated with fluorogenic substrates. The reaction is quantitated by fluorescence. The method is compatible with water-insoluble substrates (lipases), water-soluble substrates (glycosidases), whole-protein substrates (proteases), and enzyme inhibition measurements. Hydrolytically labile umbelliferyl esters can be used to assay lipases in this format without background hydrolysis. High throughput and reproducibility were tested by fingerprint analysis of lipases and esterases against 37 different fluorogenic ester substrates. A set of eight fluorogenic unbelliferyl esters was selected for optimal activity screening of lipases and esterases on silica gel plates.