178420-75-2Relevant articles and documents
NUCLEOSIDE ANALOGUE, PREPARATION METHOD AND APPLICATION
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Paragraph 0165; 0166, (2020/09/22)
Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): wherein L1, L2, and L3 are each independently a covalent bond or a covalently linked group; B is a base or a base derivative selected from purines, pyrimidines, or analogs thereof; R1 is —OH, a phosphate group, or a nucleotide; R2 as H or a cleavable group; R3 is a detectable group or a targeting group; R5 is an inhibitory group; R4 is H, —NH2, or —OR6, wherein R6 is H or a cleavable group; and C is a cleavable group or a cleavable bond.
Fluorescently-labeled nucleotide as well as preparation method and application thereof
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Paragraph 0133; 0137; 0138, (2018/11/22)
The invention discloses fluorescently-labeled nucleotide. The fluorescently-labeled nucleotide has a structure represented as the formula I in the description. Stable covalent bonding is formed between dNTP (deoxyribonucleoside triphosphate) and fluorescent dye molecules represented as the formula Q, and the fluorescently-labeled nucleotide has high stability in detection environments such as serum and the like, and is high in biocompatibility and suitable for detecting nucleic acid molecules in and out of cells. Due to large Stokes shift of the fluorescent dye molecules, the fluorescently-labeled nucleotide further has the advantages of good fluorescent stability, high fluorescent quantum yield and high signal-to-noise ratio of an imaging result. The invention further discloses a preparation method of the fluorescently-labeled nucleotide. The compound with the structure shown as the formula I is prepared by a click chemical reaction, reaction conditions are mild, operation is simple,reaction selectivity is high, and the fluorescently-labeled nucleotide with high yield can be prepared.
Modified nucleotides for polynucleotide sequencing
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Paragraph 0158, (2015/11/16)
The invention provides a kit comprising four modified nucleotide triphosphate molecules, each comprising a purine or pyrimidine base and a deoxyribose sugar moiety wherein the 3' carbon atom of the sugar moiety has attached a group of the structure???????? -O-Zwherein Z is of the formula -CH2N3.