185318-75-6Relevant academic research and scientific papers
Novel derivatization of protein thiols with fluorinated fluoresceins
Gee, Kyle R.,Sun, Wei-Chuan,Klaubert, Dieter H.,Haugland, Richard P.,Upson, Rosalyn H.,Steinberg, Thomas H.,Poot, Martin
, p. 7905 - 7908 (1996)
Fluorescein diacetate analogs 3a-3c are shown to react selectively with protein sulfhydryl groups at neural pH in aqueous solution, by means of substitution at the perfluorinated ring. The acetate moieties are cleaved by treatment with aqueous carbonate or by endogenous esterases, giving a fluorescent protein conjugate.
Fluorinated xanthene derivatives
-
, (2008/06/13)
The family of dyes of the invention are fluoresceins and rhodols that are directly substituted on one or more aromatic carbons by fluorine. These fluorine-substituted fluorescent dyes possess greater photostability and have lower sensitivity to pH changes in the physiological range of 6-8 than do non-fluorinated dyes, exhibit less quenching when conjugated to a substance, and possess additional advantages. The dyes of the invention are useful as detectable tracers and for preparing conjugates of organic and inorganic substances.
