186032-65-5 Usage
Uses
Used in Molecular Biology and Genetic Research:
5-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester is used as a probe in fluorescent dye labeled terminators for DNA sequencing. This application is crucial for determining the order of nucleotides within a DNA molecule, which is essential for understanding genetic information, diagnosing genetic disorders, and advancing personalized medicine.
The use of 5-CARBOXYFLUORESCEIN DIPIVALATE N-HYDROXYSUCCINIMIDE ESTER in DNA sequencing allows for the real-time monitoring of the incorporation of nucleotides during the polymerase chain reaction (PCR) process. As the DNA polymerase enzyme adds nucleotides to the growing DNA strand, the fluorescent signal from the 5-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester labeled terminators increases, providing a visual representation of the DNA sequence.
In addition to its role in DNA sequencing, 5-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester can also be used in other molecular biology applications, such as:
Used in Biomedical Research:
In this industry, 5-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester is used as a fluorescent label for various biological molecules, including proteins, peptides, and nucleic acids. This allows researchers to track and visualize the behavior of these molecules within living cells or organisms, providing valuable insights into their function and interactions.
Used in Drug Discovery and Development:
5-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester is employed as a tool in high-throughput screening (HTS) assays, which are used to identify potential drug candidates that can modulate the activity of specific biological targets. The fluorescent properties of 5-CARBOXYFLUORESCEIN DIPIVALATE N-HYDROXYSUCCINIMIDE ESTER enable the rapid and sensitive detection of target interactions, accelerating the drug discovery process.
Used in Environmental Monitoring:
In the field of environmental science, 5-Carboxyfluorescein Dipivalate N-Hydroxysuccinimide Ester can be used as a tracer to study the transport and fate of pollutants in aquatic systems. Its fluorescent signal allows for the detection of even low concentrations of contaminants, helping to assess the impact of human activities on the environment and inform remediation strategies.
Check Digit Verification of cas no
The CAS Registry Mumber 186032-65-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,8,6,0,3 and 2 respectively; the second part has 2 digits, 6 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 186032-65:
(8*1)+(7*8)+(6*6)+(5*0)+(4*3)+(3*2)+(2*6)+(1*5)=135
135 % 10 = 5
So 186032-65-5 is a valid CAS Registry Number.
186032-65-5Relevant academic research and scientific papers
The fluorescence signal for increasing the use of divalent metal
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Paragraph 0177; 0179, (2021/08/21)
Compositions comprising fluorescent or colored dyes and methods of using the same in combination with divalent metal salts to analyze samples are disclosed.
NEW TECHNOLOGY TO CONJUGATE THE TACCALONOLIDE MICROTUBULE STABILIZERS WITH LINKERS/PAYLOADS
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Paragraph 0506, (2020/09/27)
The present disclosure is concerned with taccalonolide analogs and conjugated taccalonolide analogs useful as cellular probes and in the treatment of, for example, hyperproliferative disorders such as cardiovascular diseases and cancer. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
ULTRA BRIGHT DIMERIC OR POLYMERIC DYES WITH SPACING LINKER GROUPS
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Paragraph 0410; 0412, (2017/11/01)
Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I): or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M, m and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.
COMPOSITIONS COMPRISING A POLYMERIC DYE AND A CYCLODEXTRIN AND USES THEREOF
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Page/Page column 129, (2017/12/01)
Compositions comprising a polymeric dye and a cyclodextrin are provided. Methods associated with preparation and use of such compounds, for example in methods for detection of any analyte molecule, are also provided.
