187539-25-9Relevant academic research and scientific papers
Improved impurity profile of phosphorothioate oligonucleotides through the use of dimeric phosphoramidite synthons
Krotz, Achim H.,Klopchin, Patrick,Cole, Douglas L.,Ravikumar, Vasulinga T.
, p. 1637 - 1640 (1997)
Phosphorothioate oligonucleotides synthesized through assembly of dimeric phosphoramidite synthons show a significantly improved impurity profile compared to oligomers synthesized through coupling of standard monomer phosphoramidites. A greater than 70% reduction of the (n-1)-mer population and a ca. 50% reduction of phosphodiester linkages has been achieved.
Reactivity of 3H-1,2,4-dithiazole-3-thiones and 3H-1,2-dithiole-3-thiones as sulfurizing agents for oligonucleotide synthesis
Guzaev, Andrei P.
supporting information; experimental part, p. 434 - 437 (2011/02/28)
The reactivity of 5-amino-3H-1,2,4-dithiazole-3-thiones substituted at their amino group and 5-amino-3H-1,2-dithiole-3-thiones substituted at their amino group and C4 toward compounds containing P(III) atoms has been studied. N,N-Disubstituted-N′-(3-thioxo-3H-1,2,4-dithiazol-5-yl)methanimidamides were selected as novel efficient sulfur transfer reagents suitable for DNA and RNA synthesis.
High-yield solution-phase synthesis of di- and trinucleotide blocks assisted by polymer-supported reagents
Dueymes, Cecile,Schoenberger, Andreas,Adamo, Ilaria,Navarro, Aude-Emmanuelle,Meyer, Albert,Lange, Meinolf,Imbach, Jean-Louis,Link, Fritz,Morvan, Francois,Vasseur, Jean-Jacques
, p. 3485 - 3488 (2007/10/03)
(Chemical Equation Presented) A new solution-phase phosphoramidite approach is reported for oligonucleotide synthesis employing recyclable solid-supported reagents. It uses polyvinyl pyridinium tosylate as the activator of a nucleoside-3′-O-phosphoramidit
Investigation of a facile synthetic method for phosphorothioate dimer synthons in oligonucleotide phosphorothioates synthesis
Miyashita, Takanori,Yamada, Kohei,Kondo, Kazuhiko,Mori, Kenya,Shinozuka, Kazuo
, p. 955 - 962 (2007/10/03)
A facile synthetic method of a phosphorothioate dimer block was investigated. Dinucleoside phosphite triester intermediates were obtained in one-pot synthesis by the coupling of a protected nucleoside bearing free 5'- OH and a protected nucleoside bearing free 3'-OH in the presence of phosphorous trichloride (PCl3) and 1,2,4-triazole. The intermediates were easily sulfurized to afford the desired phosphorothioate dimer blocks in 33- 64% overall yields.
Synthesis of dimer phosphoramidite synthons for oligodeoxyribonucleotide phosphorothioates using diethyldithiocarbonate disulfide as an efficient sulfurizing reagent
Eleuteri, Alessandra,Cheruvallath, Zacharia S.,Capaldi, Daniel C.,Cole, Douglas L.,Ravikumar, Vasulinga T.
, p. 1803 - 1807 (2007/10/03)
An efficient solution phase synthesis of deoxyribonucleoside phosphorothioate dimers utilizing phosphoramidite approach is described. Diethyldithiocarbonate disulfide (DDD) was found to be an efficient sulfurizing reagent in the conversion of phosphite triesters to phosphorothioate triesters.
