Improved impurity profile of phosphorothioate oligonucleotides through the use of dimeric phosphoramidite synthons

Article abstract of DOI:10.1080/07328319708006244

Phosphorothioate oligonucleotides synthesized through assembly of dimeric phosphoramidite synthons show a significantly improved impurity profile compared to oligomers synthesized through coupling of standard monomer phosphoramidites. A greater than 70% reduction of the (n-1)-mer population and a ca. 50% reduction of phosphodiester linkages has been achieved.

Full text of DOI:10.1080/07328319708006244

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Nucleosides and Nucleotides
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Improved Impurity Profile of
Phosphorothioate Oligonucleotides
Through the Use of Dimeric
Phosphoramidite Synthons
Achim H. Krotz a , Patrick Klopchin a , Douglas L. Cole a &
Vasulinga T. Ravikumar a
a Isis Pharmaceuticals , 2292 Faraday Avenue, Carlsbad, CA,
92008, USA Fax: E-mail:
Published online: 16 Aug 2006.
To cite this article: Achim H. Krotz , Patrick Klopchin , Douglas L. Cole & Vasulinga T. Ravikumar
(1997) Improved Impurity Profile of Phosphorothioate Oligonucleotides Through the Use of
Dimeric Phosphoramidite Synthons, Nucleosides and Nucleotides, 16:7-9, 1637-1640, DOI:
10.1080/07328319708006244
To link to this article: http://dx.doi.org/10.1080/07328319708006244
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