187610-68-0Relevant academic research and scientific papers
Cleavage of β-lactone ring by serine protease. Mechanistic implications
Kim, Dong H.,Park, Jeong-il,Chung, Sang J.,Park, Jung Dae,Park, No-Kyung,Han, Jong Hoon
, p. 2553 - 2560 (2007/10/03)
Both enantiomers of 3-benzyl-2-oxetanone (1) were found to be slowly hydrolyzed substrates of α-chymotrypsin having kcat values of 0.134±0.008 and 0.105±0.004 min-1 for (R)-1 and (S)-1, respectively, revealing that α-CT is virtually unable to differentiate the enantiomers in the hydrolysis of 1. The initial step to form the acyl-enzyme intermediate by the attack of Ser-195 hydroxyl on the β-lactone ring at the 2-position in the hydrolysis reaction may not be enzymatically driven, but the relief of high ring strain energy of β-lactone may constitute a major driving force. The deacylation step is also attenuated, which is possibly due to the hydrogen bond that would be formed between the imidazole nitrogen of His-57 and the hydroxyl group generated during the acylation in the case of (R)-1, but in the α-CT catalyzed hydrolysis of (S)-1 the imidazole nitrogen may form a hydrogen bond with the ester carbonyl oxygen.
Amide Bond Replacements Incorporated into CCK-B Selective "Dipeptoids"
Fincham, Christopher I.,Higginbottom, Michael,Hill, David R.,Horwell, David C.,O'Toole, John C,et al.
, p. 1472 - 1484 (2007/10/02)
This paper describes the chemical synthesis and CCK-B and CCK-A receptor binding affinities of a series of compounds in which the central amide bond of the CCK-B "dipeptoid" ligand tricyclo3,7>dec-2-yl-21-(hydroxymethyl)-2
