18771-50-1 Usage
Uses
Used in Pharmaceutical Industry:
Tetrahydrouridine is used as a research compound for studying the role of CDA in various diseases, including cancer and mitochondrial DNA depletion syndrome.
Used in Cancer Treatment:
Tetrahydrouridine is used as a potential and promising treatment for tumors with highly expressed CDA. It inhibits cell proliferation through cell cycle regulation, regardless of CDA expression levels.
Used in Combination Therapy:
Tetrahydrouridine is used in combination with the DNA methyltransferase inhibitor 5-fluoro-2'-deoxycytidine, which is being evaluated in animal models and clinical trials for diseases, including cancer and mitochondrial DNA depletion syndrome. This combination therapy aims to enhance the therapeutic outcomes and overcome the limitations of individual treatments.
Laboratory Procedures
Tetrahydrouridine is supplied as a crystalline solid. A stock solution may be made by dissolving the tetrahydrouridine in the solvent of choice. Tetrahydrouridine is soluble in organic solvents such as DMSO and dimethyl formamide, which should be purged with an inert gas. The solubility of tetrahydrouridine in these solvents is approximately 10 and 16 mg/ml, respectively.
Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of tetrahydrouridine can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of tetrahydrouridine in PBS, pH 7.2, is approximately 5 mg/ml. We do not recommend storing the aqueous solution for more than one day.
Check Digit Verification of cas no
The CAS Registry Mumber 18771-50-1 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,8,7,7 and 1 respectively; the second part has 2 digits, 5 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 18771-50:
(7*1)+(6*8)+(5*7)+(4*7)+(3*1)+(2*5)+(1*0)=131
131 % 10 = 1
So 18771-50-1 is a valid CAS Registry Number.
InChI:InChI=1/CH4N2S/c2-1(3)4/h(H4,2,3,4)
18771-50-1Relevant academic research and scientific papers
Organic compounds and process
-
, (2008/06/13)
Novel hydrogenated pyrimidine nucleosides and nucleotides are produced by catalytic hydrogenation, particularly in the presence of a rhodium catalyst. The novel compounds are specifically used to inhibit deaminating enzymes, which would inactivate cytosine arabinoside by conversion to uridine arabinoside. Cytosine arabinoside is used for its anti-viral, particularly anti-herpes and anticytotoxic activity in mammals and birds, as well as to destroy phages which interfere with the production of antibiotics. Novel formulations containing cytosine arabinoside and the hydrogenated pyrimidine nucleosides are advantageous to provide prolonged cytosine arabinoside effects.
