189373-41-9

Usage

Uses

Used in Biochemical Research:
DBD-ED is used as a fluorescence derivatization reagent for enhancing the detection of various biomolecules in biochemical research. Its application reason is to improve the sensitivity and specificity of analytical techniques, such as high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE), by providing a highly fluorescent tag to the target molecules.
Used in Pharmaceutical Analysis:
In the pharmaceutical industry, DBD-ED is used as a derivatization agent for the analysis of drugs and their metabolites. The application reason is to facilitate the identification and quantification of active pharmaceutical ingredients (APIs) and their metabolites in biological samples, such as blood, urine, and tissue homogenates, which is crucial for drug development, quality control, and pharmacokinetic studies.
Used in Environmental Monitoring:
DBD-ED is also used as a precolumn derivatization reagent for the detection and quantification of environmental pollutants and contaminants. The application reason is to improve the sensitivity and accuracy of environmental monitoring methods, allowing for the detection of trace amounts of pollutants in water, soil, and air samples, which is essential for assessing environmental quality and ensuring public health.
Used in Food Analysis:
In the food industry, DBD-ED is used as a fluorescence derivatization reagent for the analysis of additives, contaminants, and other components in the food matrix. The application reason is to enhance the detection and quantification of these components, enabling the assessment of food quality, safety, and compliance with regulatory standards.

InChI:InChI=1/C10H15N5O3S/c1-15(2)19(16,17)8-4-3-7(12-6-5-11)9-10(8)14-18-13-9/h3-4,12H,5-6,11H2,1-2H3

Upstream product

• 142246-48-8 7-chlorobenzo[c][1,2,5]oxadiazole-4-sulfonic acid chloride 
• 7116-16-7 4-chloro-2,1,3-benzoxadiazole 
• 192119-42-9 7-chloro-N,N-dimethylbenzo[c][1,2,5]oxadiazole-4-sulfonamide 
• 107-15-3 ethylenediamine 

Relevant academic research and scientific papers

Multiplexing sensory molecules map protons near micellar membranes

(Figure Presented) Hi-fi mapping: Multiplexing fluorescent sensors that simultaneously target proton concentration and polarity move to micellar nanospaces, self-regulate their positions, and report their pKa values and wavelengths, which are c

Discovery of Nonpeptide, Environmentally Sensitive Fluorescent Probes for Imaging p53-MDM2 Interactions in Living Cell Lines and Tissue Slice

Based on structural optimization work, probes 9-11 with practical activity and selectivity in tissue as well as living cell lines are well designed and synthesized. All the probes showed potent inhibitory and acceptable cell toxicity compared with the com

Environment-sensitive turn-on fluorescent probes for p53-MDM2 protein-protein interaction

A series of probes with a turn-on switch for the p53-MDM2 protein-protein interaction were developed. After careful evaluation, these small molecule fluorescent probes exhibited high practical activity and selectivity in vitro and in cellulo. In particular probe 10, which had a Ki value of 0.03 μM, displayed much better binding affinity compared to the positive control Nutlin-3, which had a Ki value of 0.23 μM. These no-wash environment-sensitive turn-on fluorescent probes have been successfully applied to imaging p53-MDM2 interaction in the human lung cancer cell line A549 (wild-type p53) at the micromolar level. Therefore, these fluorescent probes are expected to be used in drug screening and cell staining in p53-MDM2 fields, as well as in pathological and physiological studies of the p53-MDM2 interaction.