189373-41-9Relevant articles and documents
Multiplexing sensory molecules map protons near micellar membranes
Uchiyama, Seiichi,Iwai, Kaoru,De Silva, A. Prasanna
, p. 4667 - 4669 (2008)
(Figure Presented) Hi-fi mapping: Multiplexing fluorescent sensors that simultaneously target proton concentration and polarity move to micellar nanospaces, self-regulate their positions, and report their pKa values and wavelengths, which are c
Environment-sensitive turn-on fluorescent probes for p53-MDM2 protein-protein interaction
Liu, Tingting,Jiang, Yan,Liu, Zhenzhen,Li, Jin,Fang, Kun,Zhuang, Chunlin,Du, Lupei,Fang, Hao,Sheng, Chunquan,Li, Minyong
supporting information, p. 1668 - 1672 (2017/08/22)
A series of probes with a turn-on switch for the p53-MDM2 protein-protein interaction were developed. After careful evaluation, these small molecule fluorescent probes exhibited high practical activity and selectivity in vitro and in cellulo. In particular probe 10, which had a Ki value of 0.03 μM, displayed much better binding affinity compared to the positive control Nutlin-3, which had a Ki value of 0.23 μM. These no-wash environment-sensitive turn-on fluorescent probes have been successfully applied to imaging p53-MDM2 interaction in the human lung cancer cell line A549 (wild-type p53) at the micromolar level. Therefore, these fluorescent probes are expected to be used in drug screening and cell staining in p53-MDM2 fields, as well as in pathological and physiological studies of the p53-MDM2 interaction.