197238-45-2Relevant articles and documents
Oligonucleotide synthesis by the use of a 2-(levulinyloxymethyl)-5-nitrobenzoyl group as the novel base-labile protecting group for the 5'-hydroxyl groups of ribonucleoside and 2'-deoxyribonucleoside 3'-phosphoramidites
Kamaike, Kazuo,Takahashi, Hiroaki,Kakinuma, Takashi,Morohoshi, Kimio,Ishido, Yoshiharu
, p. 6857 - 6860 (2007/10/03)
A novel 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) protecting group for the 5' position of nucleoside 3'-phosphoramidites was successfully applied to the solid-phase synthesis of both an oligodeoxyribonucleotide (TpTpT and TpTpTpT) and an octaribonucleotide in combination with a 2'-O-Thp protecting group (UpCpApGpUpUpGpG). The LMNBz group was simply unmasked due to its base-labile property by a two-step procedure, i.e., treatments with 0.5 M hydrazine hydrate in 1:4 acetic acid - pyridine, and then with 0.5 M imidazole in acetonitrile.