197722-93-3Relevant academic research and scientific papers
Pyrene chromophores for the photoreversal of psoralen interstrand crosslinks
Stadler, Jens M.,Stafforst, Thorsten
, p. 5260 - 5266 (2014/07/08)
Applying psoralen interstrand crosslinks for the photoactivation of nucleic acids is a new concept. To find chromophores that can efficiently stimulate crosslink repair we screened several pyrenes and appended them to peptide nucleic acids for their site-selective addressing. Even though pyrenes conjugated to uracil revealed desirable spectroscopic properties they were not effective in crosslink reversal. In contrast, bare pyrenes are well suitable for crosslink repair with 350 nm light showing an uncaging efficiency similar to classical photocaging groups.
A new reagent for labeling macromolecules: An activated derivative of the pyrene bichromophore with excimer fluorescence
Balakin,Korshun,Prokhorenko,Maleev,Kudelina,Gontarev,Berlin
, p. 28 - 36 (2007/10/03)
N-[2-(1-pyrenyl)ethyl]-1-pyrenylacetamide, bis[2-(1-pyrenyl)ethyl]amine, and N,N-bis[2-(1-pyrenyl)ethyl]succinamide were synthesized from 1-pyrenylacetic acid. These compounds contain adjacent pyrene residues and display excimer fluorescence. The latter compound, as a pentafluorophenyl ester, was used to prepare fluorescently labeled oligodeoxyribonucleotide (5)CAGGAAACAGCTATGAC. For N,N-bis[2-(1-pyrenyl)ethyl]succinamide, the excimer-to-monomer fluorescence ratio and intensity of fluorescence in water-methanol solutions changed in the presence of single-stranded and double-stranded oligonucleotides, upon attachment to an oligonucleotide, and upon hybridization of the resulting conjugate with the complementary nucleotide sequence.
New pyrene derivatives for fluorescent labeling of oligonucleotides
Korshun,Prokhorenko,Gontarev,Skorobogatyi,Balakin,Manasova,Malakhov,Berlin
, p. 1461 - 1464 (2007/10/03)
A series of pyrene-containing reagents have been synthesized and used for the fluorescent labeling of oligonucleotides.
