2021-84-3Relevant articles and documents
Creation of an α-mannosynthase from a broad glycosidase scaffold
Yamamoto, Keisuke,Davis, Benjamin G.
supporting information; experimental part, p. 7449 - 7453 (2012/09/21)
α-Mannosides made easy: Mutation of a family-GH31 α-glucosidase that displays plasticity to alterations at the 2-OH position of donor substrates created an efficient α-mannoside-synthesizing biocatalyst. A simple fluoride donor reagent was used for the synthesis of a range of mono-α-mannosylated conjugates using the α-mannosynthase displaying low (unwanted) oligomerization activity. Copyright